Evonik Degussa Gmbh

Codexis, Inc.

Evonik Operations Gmbh

Technische Universitat Munchen

Hans-Georg Joerg Hennemann

Steffen Schaffer

Thomas Haas

Steven James Collier

Oscar Alvizo

Mirja Wessel

Seong Ho Oh

Wenjuan Zha

Harald Haeger

Markus Poetter

Martin Roos

Jan Christoph Pfeffer

Frank Erhardt

Markus Pötter

Jasmin Gielen

Arne Skerra

Oliver Thum

Wolfgang Kroutil

Thomas Daussmann

Harald Häger

Andreas Schmid

Wilfried Blümke

Jasmin Corthals

Heiko Andrea

Ralf Kelle

Michael Volland

Bruno Bühler

Eva-Maria Eckl

Sabine Hafkemeyer

Mattijs Kamiel Julsing

Manfred Schrewe

Sjef Cornelissen

Lukas Falke

Silvana Roeder

Wilfried Bluemke

Michaela Hauberg

Katharina Berse

Marion Schiebelhut

Harald Hager

Bruno Buehler

Markus PÖTTER

The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.

Ketoreductase polypeptides for the preparation of phenylephrine

The invention provides a process for reacting a carboxylic acid ester of the formula (I)R-A-COOR&#x2003;&#x2003;(I),

Process for reacting a carboxylic acid ester

The present invention relates to a method for removing an organic compound from an aqueous solution, comprising the steps of providing the aqueous solution which contains the organic compound, and a hydrophobic organic solution, where the latter comprises a liquid hydrophobic cation exchanger, contacting the aqueous solution and the hydrophobic organic solution, and separating off the hydrophobic organic solution from the aqueous solution, wherein the liquid hydrophobic cation exchanger is a saturated alkanoic acid having at least one alkyl substituent, where the organic compound is an organic compound having at least one positive charge and a neutral or positive total charge.

Branched-chain fatty acids as liquid cation exchangers

A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.

Method for aerobically producing alanine or a compound produced using alanine

The invention relates to a whole-cell catalyst which expresses a recombinant &#x3b1;-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the &#x3b1;-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an &#x3b1;-dioxygenase and contacting the product with a transaminase.

Producing amines and diamines from a carboxylic acid or dicarboxylic acid or a monoester thereof

The invention provides a whole cell catalyst which expresses a recombinant &#x3b1;-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the &#x3b1;-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a &#x3b1;-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

Production of omega-amino fatty acids

The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C &#x2014;(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

Enzymatic omega-oxidation and omega-amination of fatty acids

A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of &#x3b2;-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the &#x3b2;-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium

The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.

Biocatalytic oxidation process with AlkL gene product

The invention relates to genetically engineeredcells, use thereof and a method of production of &#x3c9;-hydroxycarboxylic acids and &#x3c9;-hydroxycarboxylic acid esters.

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Growing community of inventors

Bedburg, Germany

Hans-Georg Joerg Hennemann