Cytiva Sweden Ab

Ge Healthcare Bio-sciences Ab

Ge Healthcare Bio-sciences Corp.

Biotage Ab

Hanno Ehring

Klaus Gebauer

Erik Nils Ostlund

Mats Lundkvist

Andreas Marcstrom

Henrik Svanberg

David Carl Martin Bergman

Radislav Alexandrovich Potyrailo

Ali Can

Siavash Yazdanfar

Cheryl Margaret Surman

Lars C Andersson

Ying Mao

Vincent Francis Pizzi

Owe Salven

Anders Wilen

Dan Hermansson

Andrew David Pris

Mikael Berg

Helena Skoglar

Mikael Hornqvist

David Bergman

Esmaeil Heidari

Kine Frej

Peter Hagerlid

Mats Rimmo

Mikael Anders Hornqvist

Joakim Lundkvist

Pierre Le Greves

Hans Blom

Mircea Georgescu

Joakim Svensson

Björn Ekström

Owe Salvén

Linda Mathiasson

Jozsef Vasi

Sam Petrocelli

Zhangyi Zhong

Fredrik Lars Jarhede

Esfir Lofman

Rebecka Chmielowski

Esfir Lovman

Andreas MARCSTRÖM

Esfir LÖFMAN

Vincent F Pizzi

Anders WILÉN

Disclosed is an optical flow cell and a method for bioprocessing applications. The optical flow cell comprises a fluid inlet, a fluid outlet, and a fluid flow channel provided between said fluid inlet and said fluid outlet. The optical flow cell also comprises an output optical waveguide configured to emit light into said fluid flow channel, and a collector optical waveguide to collect light from said fluid flow channel. An optical path-length adjuster for varying the optical pathlength between said output optical waveguide and said collector optical waveguide is also provided.

Optical flow cell for bioprocessing

A method and instrument for determining optical density of a solution is disclosed. A flow cellhaving at least three light paths () is provided (), wherein each light path has a respective predetermined path length, l. Absorbance readings are taken (), A, of the solution at the at least three light paths (). For each pair of light paths, a slope, αc, is calculated () by dividing a difference in absorbance reading, ΔA, with a difference in path length, Δl. The calculated slopes, αc, are compared (), and a) if the calculated slopes, αc, are the same, the slope is used for determining () optical density of the solution, or b) if the calculated slopes, αc, are not the same, the steepest slope of the calculated slopes is used for determining () optical density of the solution, or the slope of the calculated slopes being in the range of an absorbance reading of 0.01 to 2 is used for determining () optical density of the solution.

Method and apparatus for determining optical density of a solution

An apparatus for measuring the absorbance of a substance in a solution includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

Apparatus and method for measuring the light absorbance of a substance in a solution

A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.

Detection of foam levels

An optical measuring device () configured for being connected in a flow path, said optical measuring device () comprising:—a first and a second flow path connector ();—at least one flow cell part () provided in between the first and second flow path connectors () such that a flow in a flow path in which the optical measuring device () can be arranged will flow through the first flow path connector (), the at least one flow cell part () and through the second flow path connector (); and—at least one releasable connection device () arranged for releasably connecting the at least one flow cell part (3) in between the first and second flow path connectors ().

Optical flow cell

The present invention relates to an optical flow cell for a measuring device, having an input light guide with a light exit surface, an output light guide with a light entrance surface, said input light guide and output light guide being integrated with a holder to form an optical flow cell, and wherein the holder extends along a first axis and has a through hole for receiving a flow of a sample fluid, said through hole being transversal to said first axis, and the input light guide and output light guide further are arranged in said holder so that the light exit surface and the light entrance surface extend into said through hole and are arranged to be in optical alignment with each other and at a first distance from each other. The invention also relates to a measuring device having at least one optical flow cell.

Optical flow cell for an optical measuring device

The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.

An apparatus for measuring the absorbance of a substance in a solution, includes at least one sample cell arranged to contain the solution that is at least partially transparent to light of a predefined wavelength spectrum, at least two light passages through the at least one sample cell, each of the light passages having a known path length, an LED light source arrangement including at least two LEDs, each arranged to emit a light output with a wavelength within the predefined wavelength spectrum. A plurality of optical fibers, one for each light passage, is arranged at each LED for receiving the light output and guiding it to the light passages. A method for measuring the absorbance of a substance in a solution includes providing the LED light source arrangement with an associate fiber bundle for each LED.

Disclosed is an optical flow cell (') comprising: a housing () forming; an enclosed and elongated fluid channel () arranged along a first axis (); a first light guide () and a second light guide () generally concentrically arranged along a second axis () and on opposite side walls of the fluid channel, said first and second light guides having ends () removed in situ to provide a sensing gap (d).

The present invention relates to an optical flow cell () for a measuring device, having an input light guide with a light exit surface, an output light guide with a light entrance surface, said input light guide and output light guide being integrated with a holder () to form optical flow cell (), and wherein the holder () extends along a first axis (A) and has a through hole () for receiving a flow of a sample fluid, said through hole () being transversal to said first axis (A), and the input light guide and output light guide further are arranged in said holder () so that the light exit surface and the light entrance surface extend into said through hole () and are arranged to be in optical alignment with each other and at a first distance from each other. The invention also relates to a measuring device having at least one optical flow cell ().

The present invention relates to a flow cell () comprising a fluid inlet () and a fluid outlet () separated by a sample flow-through chamber () comprising at least one UV-transparent window ('), wherein the at least one UV-transparent window (&#x2032;) is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

Polymeric device suitable for ultraviolet detection

Disclosed is apparatus () for measuring fluorescence and absorbance of a substance in a sample, said apparatus () comprising: a flow cell () for containing a sample, a first light source (), a first conductor () for transmitting light from the first light source () to the flow cell () for irradiating a sample contained therein, a second conductor () for transmitting light from the flow cell () to a sample detector () arranged to detect an electromagnetic radiation that has passed through said cell (), and a processing unit () arranged to receive a first signal () from a reference detector () and a second signal () from the sample detector () and to determine an absorbance based on said first and second signals (), said apparatus () further comprising a second light source (), a third conductor () for transmitting light from the second light source () to the cell () and wherein the sample detector () is further arranged to also detect fluorescence signals in the light that has passed through the flow cell (). The invention also relates to a method for measuring the absorbance and the fluorescence of a substance in a sample.

Intergration of fluorescence detection capability into light absorbance measurement apparatus

Disclosed is an optical fiber arrangement for inducing coupling among propagation modes of light, said arrangement comprising a multimode optical fiber () having an input end () for receiving light and an output end () for emitting light, with a coupling inducing section () extending from said input end to said output end, and a holder () on which the optical fiber is arranged, wherein said multimode optical fiber has a non-circular cross section. Disclosed also is a system for measuring the absorption or determining the concentration of a substance, said system comprising at least one optical fiber arrangement.

Optical fiber arrangement for a system for measuring the light absorption or determining the concentration of a substance

The present invention relates to a flow cell () comprising a fluid inlet () and a fluid outlet () separated by a sample flow-through chamber () comprising at least one UV-transparent window (), wherein the at least one UV-transparent window () is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

An attachment arrangement for providing a sensor in a flexible bag, said attachment arrangement comprising: a sensor holding part adapted to be integrated with the flexible bag and comprising first locking part protruding out form the flexible bag, the sensor holding part holding an optical sensor inside the flexible bag; an adaptor part provided outside the flexible bag and adapted to hold an optical fiber, the adaptor part comprising second locking part adapted to mate with the first locking part to lock the first locking part and the second locking part together such that the optical fiber can communicate with the optical sensor inside the bag.

Sensor attachment arrangement for flexible bags

Growing community of inventors

Uppsala, Sweden

Hanno Ehring