Roche Sequencing Solutions, Inc.

Roche Molecular Systems, Inc.

Roche Diagnostics Operations, Inc.

Hannes Kuchelmeister

Frank Bergmann

Dieter Heindl

Christoph Seidel

Dmitriy Gremyachinskiy

Andrew Trans

Peter Crisalli

Christian Wellner

Lars Hillringhaus

Hans-Peter Josel

Yann A Astier

Stephen Gordon Will

Amar Gupta

Uwe Kobold

Meng Taing

Juraj Topolancik

Martin Rempt

Igor Kozlov

Sebastian Johannes Pomplun

Simon Ferdinand Loibl

Giuseppe Prencipe

Jelena Milic

Omid Khakshoor

Alexander Nierth

Robert Hahn

Concordio Anacleto

Hannah Marie Kallewaard-Lum

Hasham Saqib

Ian Quang Chau

Nikolaus Klaus-Peter Stengele

Thomas Hoffmann

Daniela Mazzier

Sara Kelley

The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.

Benzylpyridinium reagent for mass spectrometry

The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing.

Tagged nucleoside compounds useful for nanopore detection

The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection

Molecules may be analyzed (e.g., sequencing of nucleic acid molecules) by tunneling recognition at a tunneling junction. Embodiments of the present invention may allow detecting individual nucleotides and the sequencing of a nucleic acid molecule using a tunneling junction. By labeling a specific nucleotide with a moiety, tunneling junctions may generate a signal with a suitable signal-to-noise ratio. The tunneling recognition uses a tunneling current that is mostly through the moiety rather than mostly through the nucleotide or a portion of the molecule of interest. Because a single nucleotide can be detected with a signal with a suitable signal-to-noise ratio resulting from the tunneling current passing through the moiety, embodiments of the present invention may allow for fast detection of nucleotides using a tunneling current.

Tunneling junctions for sequencing

The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.

Method for labeling of aldehyde containing target molecules

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Photocleavable nucleotide reagents with high stability

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Munich, Germany

Hannes Kuchelmeister