The Scripps Research Institute

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Dartmouth College

Hannah Turner

Andrew B Ward

Barney S Graham

Michael Gordon Joyce

Masaru Kanekiyo

Jason Scott McLellan

Hadi M Yassine

Kizzmekia S Corbett

Nianshuang Wang

Robert Kirchdoerfer

Christopher Cottrell

Jesper Pallesen

Matteo Bianchi

Lars Hangartner

Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Prefusion coronavirus spike proteins and their use

Provided herein are methods for mapping antibody binding to an immunogen, comprising: immunizing a subject with an immunogen and obtaining sera from the immunized subject at multiple time intervals following immunization, wherein the sera comprises antibodies that are used to form one or more immune complexes with the immunogen; isolating the one or more immune complexes formed by the serum derived antibodies bound to the immunogen; imaging, by electron microscopy, the one or more immune complexes in each of the time intervals, to obtain structural images formed between the immunogen and serum antibodies; determining, from the plurality of structural images, immunogen-antibody binding site for each of the immune complexes obtained at the plurality of time intervals; mapping immunogen-antibody binding by measuring differences in structural images obtained at different time intervals to determine immunogen-antibody binding over multiple time intervals.

Epitope mapping method

Growing community of inventors

La Jolla, CA, United States of America

Hannah Turner