Trizell Limited

Ark Therapeutics Limited

Ferring Ventures Ltd

Hanna P Lesch

Nigel Parker

Seppo Yla-Herttuala

Minna Hassinen

Piia Kristiina Valonen

Eva Kristiina Rasanen

Tarja Hannele Tuunanen

Minna Kristiina Karhinen

Jenni Mykkanen

Sara Paulo

Robert Shaw

Kari Juhani Airenne

Seppo Ylä-Herttuala

Hanna Leinonen

Anniina Valkama

Eevi Lipponen

Joonas Malinen

Milla Jonna Karoliina Karnaattu

Eva Kristiina RÄSÄNEN

Achim MÜLLER

Kari J Airenne

A process for assaying viral vector manufactured by large-scale viral vector manufacturing processes to assure the resulting vector has acceptable purity and potency. The process entails three different types of assays, each one of which is optionally useful on a stand-alone basis, and which together provide the first system able to assure the quality of viral vector produced by large-scale vector manufacturing processes.

Viral vector assay and vector

We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.

Method of manufacturing a recombinant polypeptide

We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and COare managed during transfection.

Large-scale PEI-mediated plasmid transfection

Fixed-bed bioreactor with constant-flow pump / tubing system

Novel forward primer, reverse primer and poly-linker suitable for replication of nucleic acids in e.g., 293 cells.

Nucleic acid application primers

Growing community of inventors

Kuopio, Finland

Hanna P Lesch