Nanjing Tech University

Nanjing University of Technology

Hanjie Ying

Yong Chen

Jinglan Wu

Dong Liu

Xiaochun Chen

Jingjing Xie

Chenjie Zhu

Jianxin Bai

Ming Li

Pingkai Ouyang

Jian Xiong

Tao Shen

Chenglun Tang

Xiao Zhang

Wei Zhuang

Zhuotao Tan

Qingguo Liu

Xiaoqing Lin

Huanqing Niu

Bin Yu

Ming Lei

Pengpeng Yang

Di Zhang

Ting Guo

Tao Zhou

Qing Li

Yaping Fu

Yaoying Han

Lingling Pu

Xinjian Xu

Jiannong Shen

Haifeng Liu

Zhiwei Chang

Lei Ji

Yixin Feng

Wenbin Qian

An Li

Yuhan Yang

Shishi Wu

Xiwei Peng

Xingyuan Cao

Lingxia Zhong

Tao Gan

Jungiang Shan

Wenyan Ji

Wujin Sun

He Song

Jiansheng Fan

The present invention discloses a fully bio-based, highly filled lignin-rubber masterbatch, a method for preparing same, and use thereof. The lignin-rubber masterbatch is prepared by a method comprising: (1) reacting a lignin, acetic acid, and oleic acid in the presence of a catalyst to give a modified lignin; and (2) blending the modified lignin and a rubber, and granulating to give the lignin-rubber masterbatch. The highly filled lignin-rubber masterbatch prepared by the present invention can replace the conventional reinforcing agent carbon black and provide a better reinforcing effect and higher mechanical properties for rubber materials. The present invention can also reduce the rubber content of the rubber composite materials while retaining the mechanical properties, thus featuring cost-efficiency.

Fully bio-based, highly filled lignin-rubber masterbatch, method for preparing same, and use thereof

A cordycepin-based derived compound with an anti-tumor effect, and a structure of the compound is as shown in formula I; a cordycepin derivative and a pharmaceutical composition thereof provided by the invention have a good anti-tumor proliferation effect; compared with a parent drug, the cordycepin derivative has better affinity to cell membranes, so that a half-life period of in-vivo metabolism of the drug is longer, and in-vivo remaining time of the drug is longer; compared with other nucleoside anti-tumor drugs, the cordycepin derivative and the pharmaceutical composition thereof provided by the invention have wider types and action ranges of tumors, have excellent inhibition effects on a gastric cancer, a pancreatic cancer, a liver cancer, a small cell lung cancer, a colorectal cancer, melanoma, an ovarian cancer and the like, and have lower side effects and better curative effects.

Cordycepin-based derivatized compound with anti-tumor effect

A method for preparing a nitrogen-containing heterocyclic compound and a derivative thereof by an enzymatic-chemical cascade method, comprising: reacting an alcohol, an amine, an alcohol dehydrogenase, a flavin molecule and a coenzyme in a solvent to obtain the nitrogen-containing heterocyclic compound and the derivative thereof; compared with the prior art, the method is a green and economical enzymatic-chemical cascade method, and is used for synthesizing nitrogen-containing heterocyclic compounds and derivatives thereof; compared with a common toxic chemical catalyst, the alcohol dehydrogenase is selected as a catalyst in the method, which has the characteristics of high substrate specificity, no pollution, high catalytic efficiency, no toxic solvents and simple post-treatment.

Method for preparing nitrogen-containing heterocyclic compound and derivative thereof by enzymatic-chemical cascade method

The invention belongs to the field of rubber tire processing, and particularly discloses an application of a lignin in preparing a radial tire, wherein the lignin is capable of completely or partially replacing resorcinol, and meanwhile, the lignin is also capable of completely or partially replacing an anti-aging agent. Compared with the prior art, the resorcinol is replaced or partially replaced by a biomass-derived lignin or a modified lignin in the invention, so that requirements of cost reduction, environmental protection and no toxicity are satisfied. Especially, the lignin modified by demethylation according to the invention is capable of effectively enhancing various properties of rubber.

Application of lignin in preparing radial tire

The invention discloses a recombinantfor producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinantis constructed by overexpressing a protease gene FtsH in a; and the construction method comprises the following steps of: (1) performing PCR on a genome of the, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the, and screening to obtain the recombinant. The invention has the beneficial effects that thefor overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of theis enhanced, such that a yield of continuous immobilized fermentation of theis increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

Recombinant corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and construction method thereof

The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant, wherein the recombinantis constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a. The recombinantcan effectively improve eDNA secretion of theand reduce eDNA degradation of the, so that thecan be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of theis increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

Method for producing lysine by utilizing adsorption and immobilized fermentation of recombinant corynebacterium glutamicum

It discloses a lignin enhanced wood-plastic composite, belonging to the field of polymer based composites. The wood-plastic composite is composed of one or more of plant fiber, lignin, a recycled thermoplastic plastic, mineral powder and a processing aid, and is processed by a screw granulating extrusion process. Compared with the prior art, the mechanical properties, heat stability and aging resistance of a wood-plastic material are improved by adding lignin into the wood-plastic material according to the present invention.

Lignin enhanced wood-plastic material and preparation method thereof

Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium.

Preparation method of yeast cell immobilization medium and application thereof

It discloses a chemical regeneration method of oxidized coenzyme NAD(P)which is under an oxygen or air atmosphere condition, adding a catalytic amount of bridged flavin, and oxidizing NAD(P)H to obtain NAD(P). The catalyst for regeneration of cofactor is cheap and easily available small organic molecule having no noble metal; this regeneration system can regenerate NADH and NADPH; this regeneration system has a wide pH range and temperature range, being applicable to various oxidation reactions catalyzed by nicotinamide-dependent oxidoreductase.

Chemical regeneration method of oxidized coenzyme NAD (P)+

It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates.

[object Object]

The invention relates to a method for the production of adenosine 3',5&#x2032;-monophosphate (cAMP) by a permeable microbial strain which uses polyols for protecting activities of enzymes and a cAMP precursor and phosphate as substrates. Glucose is used as an energy provider and metal ions and an organic solvent such as acetone are used in the medium.

Method for microbial production of cyclic adenosine 3', 5&#x2032;-monophosphate

The present invention providesand an application thereof. A preservation number of theprovided in the invention is CGMCC No. 5234. Theprovided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.

Clostridium acetobutylicum and application thereof

The present invention provides a method for desorbing and regenerating a butanol-adsorbing hydrophobic macroporous polymer adsorbent, comprising: successively eluting the hydrophobic macroporous polymer adsorbent with butanol adsorbed therein using a water soluble low-boiling-point polar solvent and water. The method provided in the present invention has a simple process, a short separation time, easy, fast and complete desorption and regeneration, low equipment investment and pollution, and reduced energy consumption, and therefore production is easy on a large scale.

Method for desorbing and regenerating butanol-adsorbing hydrophobic macroporous polymer adsorbent

Disclosed is a method for separating butanol. The method uses hydrophobic macroporous polymer adsorbent to separate butanol in a mixed solution, and the process comprises the following steps: 1) using macroporous polymer adsorbent to adsorb butanol in a mixed solution; 2) desorbing butanol from macroporous polymer adsorbent. The method is simple; the separation time is short; the efficiency of butanol recovery is high; and the separating cost is low.

Method for separating butanol

Growing community of inventors

Nanjing, China

Hanjie Ying