New England Biolabs, Inc.

Hang-Gyeong Chin

Sriharsa Pradhan

Pierre O Esteve

Jurate Bitinaite

Chaithanya Ponnaluri

Yu Brian Zheng

Geoffrey G Wilson

Richard J Roberts

Romualdas Vaisvila

Ivan R Correa, Jr

Christopher J Noren

Nan Dai

Elisabeth A Raleigh

Devora Cohen-Karni

Pierre Olivier Esteve

Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.

Analysis of chromatin using a nicking enzyme

Nucleic acids comprising &#x3b2;-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.

Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid

Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.

Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation

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South Hamilton, MA, United States of America

Hang-Gyeong Chin