Siemens Healthcare Diagnostics Gmbh

Ciba Corning Diagnostics Corp.

Siemens Medical Solutions Diagnostic

Hana Lukinsky

Say-Jong Law

Anand Natrajan

Qingping Jiang

David J Sharpe

Todd B Sells

Guohan Yang

Hartmut Schroeder

Chemiluminescent acridinium compounds are used in homogeneous assays to determine the concentration of an analyte in a sample without strong acid or strong base treatment. The chemiluminescent acridinium compounds include acridinium esters with electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus to inhibit pseudo-base formation, or acridinium sulfonamides with or without electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus.

Applications of acridinium compounds and derivatives in homogeneous assays

A novel method for conjugating haptens, ligands or luminescent labels to polynucleotides has been developed. This method involves the formation of a mixed anhydride, followed by reaction of the mixed anhydride intermediate with a nucleophilic group-containing polynucleotide in a DMF/water solvent system. The resulting compound finds usefulness especially in the gene probe area.

Efficient gene probe conjugations by an unconventional mixed anhydride

Growing community of inventors

Portland, OR, United States of America

Hana Lukinsky