Grape King Bio Ltd

Han-Hsin Chang

Chin-Chu Chen

Li-Ya Lee

Jui-Hsia Hsu

Shu-Hsing Yeh

Pei-Cheng Lin

Yen-Lien Chen

Lee-Sar Sheng

A method of preparingmycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a) culturing amycelium in a plate media at 15 to 35&#xb0; C. for 5 to 14 days; (b) inoculating the mycelium of step (a) to a flask containing liquid media and culturing it at 15 to 35&#xb0; C. with a pH of 2 to 8 for 3 days; (c) inoculating the mycelium of step (b) to a fermenter tank and culturing it at 15 to 35&#xb0; C. with a pH of 2 to 8 for 3 days, so as to obtain amycelium fermentation liquid; (d) freeze-drying and grating the fermentation liquid, so as to obtain amycelium powder; (e) extracting the powder with solvent, so as to obtain amycelium extract; and (f) drying the extract, so as to obtained themycelium active substances.

mycelium active substances, preparation method, pharmaceutical composition and application thereof

The present invention discloses themycelia, the active substances ofmycelia, its submerged fermentation product and its pharmaceutical composition for preventing, delaying or treating cataracts, and the methods for preparing the products above. Themycelia, the active substances ofmycelia, its submerged fermentation product and its pharmaceutical composition further can be prepared as a health food for preventing or delaying cataracts.

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Zhong-Li, Taiwan

Han-Hsin Chang