Seegene, Inc.

Han Bit Lee

Young Jo Lee

Jong Yoon Chun

Dae Young Kim

Young Wook Kim

Mi Hyun Jang

Sung Moon Ko

Young Yong Park

Yong Jo Lee

The present invention relates to a method for predicting the melting temperature (T) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tof an oligonucleotide in various reaction environments using the equations for Tcalculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.

Method for predicting the melting temperature of oligonucleotide

The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.

Method and device for analyzing target analyte in sample

The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reference value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.

Method for calibrating a data set of a target analyte using a normalization coefficient

The present invention relates to extraction of a signal for a target nucleic acid sequence from signals for two target nucleic acid sequences in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention using an amended reference value as well as an initial reference value can lead to increasing the detection accuracy in methods for detecting target nucleic acid sequences using different detection temperatures and reference values.

Signal extraction for a target nucleic acid sequence

The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

Analytical signal for determination of the presence of a target nucleic acid sequence

The present invention relates to the detection of a target nucleic acid sequence by a PTOCE-E (PTO Cleavage and Extension-dependent Extension) assay. The PTOCE-E assay of the present invention can reduce the non-target signal and increase the target signal as compared with the conventional PTOCE and PCE-SH methods, thereby enabling more accurate detection of the target nucleic acid sequence.

Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay

The present invention relates to a method for reducing a noise level of a data set for a target analyte in a sample. The present invention can reduce a noise level of a data set to a proper level conveniently by applying a noise-reduction ratio to the data set thereby the possibility of false positive may be reduced effectively. According to the present invention, the calibrated data set is obtained by using the noise-reduction ratio, so that the noise level of a data set is reduced without change of signal ratio between the data point in the data set.

Growing community of inventors

Seoul, South Korea

Han Bit Lee