Pfizer Corporation

Other

Pfizer Products Inc.

Hamish A McArthur

Kim Jonelle Stutzman-Engwall

Yoshihiro Katoh

Lapyuen H Lam

Richard G Wax

Takushi Kaneko

Yong-Jin Wu

Wei-Guo Su

Edmund W Hafner

Claudio D Denoya

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of. The present invention further relates to vectors, host cells, and mutant strains ofin which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

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The present invention relates to polynucleotide molecules comprising sequences encoding avec gene products, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of. AveC genes, homologs and partial homologs thereof are described for, and, respectively. The present invention further relates to vectors, host cells, and mutant strains ofin which the avec gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

The present invention relates to polynucleotide molecules comprising sequences encoding avec gene products, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of . AveC genes, homologs and partial homologs thereof are described for , S. hygroscopicus, and , respectively. The present invention further relates to vectors, host cells, and mutant strains of in which the avec gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

AveC gene product from Streptomyces hygroscopicus

13-methyl erythromycin derivatives

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of . The present invention further relates to vectors, host cells, and mutant strains of in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding the aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of The present invention further relates to vectors, host cells, and mutant strains of in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

Methods related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of,. The present invention further relates to vectors, host cells, and mutant strains of,in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

The process of this invention is directed to isolating or otherwise obtaining an olefinic macrolide producing microorganism which microorganism does not contain epoxidase enzyme activity. This invention also relates to a process for preparing said olefinic macrolide by fermenting a mutant microorganism lacking epoxidase activity, designated rosX herein, which mutant is obtained from the wild-type microorganism. This invention also relates to a rosX mutant of Micromonospora rosaria, and to any microorganism having the identifying characteristics thereof, said mutant also designated ATCC 55709. This invention also relates to a process for preparing repromicin, the compound of formula (II), ##STR1## by mutating a wild-type microorganism capable of producing rosamicin to produce a mutant microorganism lacking epoxidase activity such that repromicin is produced by said mutant microorganism.

Process for dienone macrolides

Mutants of Streptomyces avermitilis lacking ability to produce glycosylated avermectins and lacking branched-chain 2-oxo acid dehydrogenase activity, method for preparation thereof, and use thereof to produce natural and non-natural avermectin aglycones useful as parasiticides.

Growing community of inventors

Gales Ferry, CT, United States of America

Hamish A McArthur