Harvard College

Dana-farber-cancer Institute Inc.

T Cell Sciences, Inc.

Hamid Band

Jack L Strominger

Michael B Brenner

Stephen H Ip

Jonathan G Seidman

Michael S Krangel

The present invention is directed to purified polypeptides comprising the .gamma. T cell antigen receptor (TCR) polypeptide, the .delta. TCR polypeptide, a .gamma., .delta. TCR complex, or a fragment thereof containing an epitope. The invention also relates to nucleic acid sequences encoding such polypeptides, and subsequences thereof. In specific embodiments, the invention relates to nucleic acid sequences comprising variable, diversity, joining, or constant regions of the .delta. TCR gene sequence. The invention also relates to monoclonal antibodies specifically reactive with an epitope of the gamma or delta TCR polypeptides. In specific embodiments, these antibodies are reactive with the delta constant region, the delta variable region, or gamma constant region. Such antibodies can be identified by detecting co-modulation of the CD3 antigen. In another embodiment, the invention relates to compositions comprising substantially purified cells which express both a .gamma., .delta. TCR and the CD4 antigen. The invention also relates to a composition comprising cells which express a .gamma., .delta. TCR that is not associated with a CD3 complex.

Human .gamma., .delta.T cell antigen receptor polypeptides and nucleic

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Hamid Band