Grail, Inc.

Illumina Corporation

Illumina France Sarl

Hamed Amini

Tarun Kumar Khurana

M Shane Bowen

Steven Mark Barnard

Dietrich A Dehlinger

Yir-Shyuan Wu

Alex Aravanis

Maria Candelaria Rogert Bacigalupo

Poorya Sabounchi

Cyril Delattre

Arash Jamshidi

Arnaud Rival

Gordon M Cann

Bala Murali Venkatesan

Yan-You Lin

Samuel S Gross

Oliver Claude Venn

M Cyrus Maher

Srinka Ghosh

Jennifer Olivia Foley

Seyedmehdi Shojaee

Alexander P Fields

Rongsu Qi

Foad Mashayekhi

Majid Babazadeh

The present description provides a cancer assay panel for targeted detection of cancer-specific methylation patterns. Further provided herein includes methods of designing, making, and using the cancer assay panel for diagnosis of cancer.

Methylation markers and targeted methylation probe panel

Aspects of the invention include methods for preparing sequencing libraries, performing sequencing procedures that can correct for process-related errors, and identifying rare variants that are or may be indicative of cancer.

Library preparation and use thereof for sequencing based error correction and/or variant identification

Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

Inertial droplet generation and particle encapsulation

Aspects of the invention relate to methods and compositions for preparing and analyzing a single-stranded sequencing library from a double-stranded DNA (e.g., double-stranded cfDNA) sample. In some embodiments, the sample includes double-stranded DNA (dsDNA) molecules, and damaged dsDNA (e.g., nicked dsDNA) molecules. In some embodiments, the sample includes single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information, including strand-pairing and connectivity information, from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared using conventional methods.

Methods for single-stranded nucleic acid library preparation

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Menlo Park, CA, United States of America

Hamed Amini