National Institutes of Health, a Component of the US Dept. of Health & Human Services

Litton Bionetics, Inc.

Haleem J Issaq

Timothy D Veenstra

George Janini

Kenneth L Wilkens

Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are also disclosed. Apparatus and methods for conveying analyte ions from the capillaries into analytical instruments, such as a mass spectrometer, are also disclosed. The disclosed contiguous capillaries may be used to carryout electrophoresis separation and electrospray ionization of analytes. Methods for obtaining the mass spectra of macromolecular analytes at concentrations lower than previously possibly are provided using the apparatus and procedures described herein.

Contiguous capillary electrospray sources and analytical devices

An anti-radial chromatographic device is disclosed preferably comprising an inner Petri dish nested concentrically within an outer Petri dish of slightly larger diameter. The bottom surface of the inner dish is spaced from the bottom surface of the outer dish to form a solvent-containing chamber. A annular wick contained in the annular space between the two dishes extends above the tops of the dishes and conducts the solvent upward to the lower surface of a downward-facing chromatographic plate resting on the wick.

Growing community of inventors

Frederick, MD, United States of America

Haleem J Issaq