The University of Texas System

Massachusetts Institute of Technology

Hal S Alper

Gregory N Stephanopoulos

John Blazeck

Yong-Su Jin

Andrew Hill

Eric Young

Leqian Liu

Curt R Fischer

Elke Nevoigt

Kathleen Curran

Kelly Markham

Claire Palmer

Sunmi Lee

This invention relates to compositions and methods for the production of Type III polyketides using genetically modified oleaginous yeast strains, for example,

Compositions and methods for type III polyketide production in oleaginous yeast species

Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals.

Compositions and methods for lipid production

Provided herein are exogenous terminator sequences for use in fungi cell transcriptional termination.

Exogenous terminators for controlling fungal gene expression

Provided herein are compositions and methods useful for transporting xylose, arabinose and other monosaccharides, into a yeast cell.

Methods for engineering sugar transporter preferences

Provided herein are compositions and methods useful for reducing glucose inhibition in transporting xylose, arabinose and other monosaccharides, into a yeast cell.

Engineered xylose transporters with reduced glucose inhibition

The invention relates to global transcription machinery engineering to produce altered cells having improved phenotypes.

Global transcription machinery engineering

The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.

Promoter engineering and genetic control

The present invention provides engineered cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yjfP gene, or further disrupted for a gdhA, gpmB, aceE, ppc, talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Additionally, methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for gdhA, aceE, fdhF, yjiD, hnr or yjfP gene expression or any combination thereof and ackA, appY, aspC, clp, clpP, clpXP, crcB, csdA, cyaA, evgS, fdhA, fdhD, feoB, funA, glnE, glxR, gntK, hycI, lipB, lysU, modA, moeA, nadA, nuoC, nuoK, pflB, pitA, pst, pstC, pta, p-yjiD, sohA, stpA, yagR, yaiD, ybaS, ycfZ, ydeN, yebB, yedN, yfcC, ygjP, yibD, yjfP, yjhH, or yliE gene expression, or a combination thereof or cells inhibited for their expression, activity or function are disclosed. Methods of enhanced carotenoid synthesis utilizing cells genetically engineered to overexpress dxs, idi, ispFD, yjiD, rpoS, torC, appY, ydgK, yeiA, yedR, tort, arcB, yggT, purDH, yfjN or a combination thereof, or further disrupted for the above-referenced genes are disclosed. Methods for identifying genes involved in optimized production of a carotenoid, and cells disrupted for, or inhibited for the expression, activity or function of genes thus identified are described.

Gene targets for enhanced carotenoid production

The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yjfP gene, or further disrupted for a gdhA, gpmB aceE, ppc, talB or fdhF gene, or any combination thereof, or cells inhibited for their expression, activity or function are disclosed. Methods of enhanced carotenoid synthesis utilizing cells genetically engineered to overexpress dxs, idi, yjiD, rpoS, torC, appY, ydgK, yeiA, yedR, tort, arcB, yggT, purDH, yfjN or a combination thereof, or further disrupted for the above-referenced genes are disclosed. Methods for identifying genes involved in optimized production of a carotenoid, and cells disrupted for, or inhibited for the expression, activity or function of genes thus identified are described.

Growing community of inventors

Cambridge, MA, United States of America

Hal S Alper