Korea Institute of Science and Technology

Eubiologics Co., Ltd.

Hak Suk Chung

Eun Gyeong Yang

So Yeon Kim

Jinsu An

Ick Chan Kwon

Yu Hyun Ji

Dohyeon Hwang

Jin Su An

Chan Kyu Lee

Da Hui Ha

Choon Geun Lee

Ye Ram Lee

Yuhyun Ji

Provided is a method of producing monophosphoryl lipid A (MPLA). According to the method, MPLA may be produced with high purity and high purity by using a bacterium producing MPLA.

Method for producing monophosphoryl lipid A

Provided are a method of screening an inhibitor of caspase activity by lipopolysaccharide and a method of screening a therapeutic agent for inflammatory diseases or sepsis using the same. Accordingly, it is possible to develop a caspase-4-specific inhibitor.

Method of screening inhibitor of caspase activity by lipopolysaccharide

Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc.

Method of producing monomeric card in bacteria

A bacterium that constitutively produces monophosphoryl lipid A (MLA) and a method of producing MLA by using the bacterium may simply produce MLA and a derivative thereof without acid hydrolysis, reduce a probability of natural mutation, and increase yields of MLA and a derivative thereof by constitutive expression of the MLA and derivative thereof.

Bacterium constitutively producing monophosphoryl lipid A and method of producing monophosphoryl lipid A by using bacterium

A bacterium producing monophosphoryl lipid A (MLA) comprising a genetic modification that increases expression of a gene encoding LpxE polypeptide and a method of producing MLA are provided. According to the present invention, MLA may be produced in a simple manner without acid hydrolysis and/or base hydrolysis.

Bacterium producing monophosphoryl lipid A and method of producing monophosphoryl lipid A by using bacterium

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Seoul, South Korea

Hak Suk Chung