Bio Verde Inc.

Bmg Inc.

Hajime Sugai

Suong-Hyu Hyon

Kazuaki Matsumura

Naoki Nakajima

Masakazu Konda

A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In embodiments, &#x3b5;-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In embodiments for foods or pharmaceuticals, the &#x3b5;-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.

Composition for cryopreservation of cells and tissues

Medical-use two-part adhesive comprising; first part comprised of an aqueous solution or a powder of aldehyde-groups-introduced alpha-glucan; and second part comprised of an aqueous solution or a powder of amino-groups-containing polymer that is formed of a polymer chain of amino-group-containing units and has a molecular weight in a range of 1000 to 20,000; as well as medical-use hydrogel resin obtained by curing the adhesive. A mixture of the first and second parts, at a time of mixing them to form the hydrogel has pH in a range of 5.0 to 8.0. In a preferred embodiment, the amino-groups-containing polymer is epsilon-poly-L-lysine produced by microorganism or by enzyme.

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Hirakata, Japan

Hajime Sugai