The Green Cross Corporation

Tao Health Life Pharma Co., Ltd.

Hajime Horii

Tadakazu Suyama

Hirofumi Arimura

Haruhide Kawabe

Hiromichi Mukai

Muneo Tsujikawa

Kaoru Kobayashi

Michio Sato

Koji Naito

Masayuki Nishida

Munehiro Noda

Satoshi Horie

Shoji Ideno

Minako Hoshi

An antibody provided by the present invention has a low reactivity with amyloid precursor proteins, and has a higher reactivity with amylospheroids than with amyloid &#x3b2; fibrils or monomeric amyloid &#x3b2;-proteins. According to the present invention, an antibody is provided that has a higher reactivity with amylospheroids than with amyloid precursor proteins, and has any one or more of the following properties: (i) a higher activity with amylospheroids than with amyloid &#x3b2; fibrils; (ii) a higher reactivity with amylospheroids than with monomeric amyloid &#x3b2;-proteins; and (iii) an activity of inhibiting neuronal cell death induced by amylospheroids.

Antibody and use thereof

A DNA sequence of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) promoter and a process for preparing a heterologous protein utilizing said DNA sequence as a promoter are disclosed, said GAP-DH promoter comprising a region upstream of an initiator codon of the GAP-DH protein up to -164 bp as a minimum unit. Since the GAP-DH promoter is small in size, a physiologically active substance can be expressed in yeasts effectively and recombination of DNA can be simplified.

Yeast promoter truncated GAP-DH

A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.

Yeast promoter and process for preparing heterologous protein

Growing community of inventors

Osaka, Japan

Hajime Horii