University of Pennsylvania

New York University

Drexel University

Panametrics, Inc.

Haim H Bau

Michael G Mauk

Changchun Liu

Xianbo Qiu

William R Abrams

Daniel Malamud

Michael Schrlau

Jinzhao Song

Eran Geva

Byong Man Kim

Yury Gogotsi

Lawrence C Lynnworth

Do Young Byun

Toan Huu Nguyen

Robert Hart

Zongyuan Chen

Frederic Bushman

Xianbo Ceiu

Karen E Ocwieja

Joseph M Grogan

Amanda W Dyson

Sudhir Ramprasad

Dafeng Chen

Rui Zhang

Jason Kwa

Michael A Riegelman

Jin O Kim

Scott Sherrill-Mix

Daneil Malamud

Provided herein are devices for monitoring nucleic acid amplification, nucleic acid amplification monitors, methods of quantifying nucleic acid amplification, methods of identifying an unknown nucleic acid molecule, and systems for monitoring nucleic acid amplification. The disclosed devices, methods, and systems comprise at least one nuclemeter, comprising at least one sample chamber and at least one reaction-diffusion conduit in fluid communication with the chamber.

Devices and methods for monitoring and quantifying nucleic acid amplification

Disclosed herein are methods for multiple stage isothermal amplification of nucleic acid comprising a first substantially isothermal amplification reaction on the nucleic acid to generate a first amplification product and at least one substantially isothermal amplification reaction on the first amplification product to generate at least one second amplification product in an amount sufficient for recovery, testing, or characterization.

Multiple stage isothermal enzymatic amplification

Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.

Compositions, kits, and methods to detect HIV virus

The present disclosure provides devices and methods using separating a fluid&#x2014;e.g., plasma or serum&#x2014;from whole blood. In some embodiments, the devices and methods use hydrophobic or superhydrophobic surfaces to encourage whole blood to contact a selective membrane that extracts the desired fluid component from the blood.

Fluid separator for point of care molecular diagnostics

A plasma separator includes a vertical chamber with a membrane or membranes partially or fully covering the internal chamber walls and forming an inner cavity to accommodate whole blood. The plasma separator further includes a plasma collection space separated from the inner cavity with one or more membranes and a plasma removal port.

Plasma separator apparatus and associated methods

Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium&#x2014;such as a lateral flow strip&#x2014;where the user may label and detect the nucleic acids.

Isothermal nucleic acid amplification reactor with integrated solid state membrane

Disclosed are integrated devices capable of performing a polymerase chain reaction within a single vessel. Also disclosed are related methods of sample analysis.

Integrated PCR reactor for cell lysis, nucleic acid isolation and purification, and nucleic acid amplication related applications

Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.

Moisture-activated self-heating analysis device

Provided are flow cell devices&#x2014;referred to as nanoaquariums&#x2014;that are microfabricated devices featuring a sample chamber having a controllable height in the range of nanometers to micrometers. The cells are sealed so as to withstand the vacuum environment of an electron microscope without fluid loss. The cells allow for the concurrent flow of multiple sample streams and may be equipped with electrodes, heaters, and thermistors for measurement and other analysis devices.

Flow cells for electron microscope imaging with multiple flow streams

Disclosed are preloaded analysis modules comprising reagents disposed within a barrier material capable of liberating the reagents, at a time advantageous to a reaction scheme, when exposed to certain activation conditions or reagents. Also disclosed are related methods for analyzing samples with such modules.

Reaction chamber having pre-stored reagents

Provided are nanoscale devices suitable for multiplexed, parallel detection of multiple analytes and methods for fabricating such devices.

Multiplexed nanoscale electrochemical sensors for multi-analyte detection

Provided are probes featuring multiple electrodes, which probes have diameters in the nanometer range and may be inserted into cells or other subjects so as to monitor an electrical characteristic of the subject. The probes may also include a conductive coating on at least one probe element to improve the probes' performance. The probes may also be used to inject a fluid or other agent into the subject and simultaneously monitor changes in the subject's electrical characteristics in response to the injection. Related methods of fabricating and of using the inventive probes are also provided.

Multiple-electrode and metal-coated probes

Provided are analysis cassettes that include removable sealing layers and deformable connector layers that place various channels, chambers, and reservoirs on the cassettes into fluid communication with one another. The cassettes are suitable for use in self-contained, immunoassay devices that may be operated in an automated fashion. Also disclosed are methods for analyzing samples by use of the disclosed cassettes.

Biodetection cassette with automated actuator

Disclosed are mechanically-actuated devices for transporting fluids within a microfluidic circuit and performing diagnostic operations on a sample. Also disclosed are related methods for performing sample analysis effected by the motion of an actuator proximate to a microfluidic system.

Growing community of inventors

Swarthmore, PA, United States of America

Haim H Bau