Genetron Health (beijing) Co., Ltd.

Chinese Academy of Medical Sciences

Hai Yan

Yuchen Jiao

Sizhen Wang

Pei Wang

Kun Chen

Chunfeng Qu

Yuting Wang

Qianqian Song

Hui Liu

Dayong Xu

Qiaosong Zheng

Xiao Shi

The present invention provides a kit for early screening of hepatocellular carcinoma, comprising a gene marker detection reagent and a protein marker detection reagent. The invention also provides a preparation method and application of the kit. The kit comprising specific gene markers and protein markers of the present invention has been demonstrated to be effective in achieving early screening of HCC in community populations, particularly in prospective studies.

Kit for early screening of liver cell cancer and preparation method and use thereof

A method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer. The library construction method and sequencing data analysis process have the following advantages: 1. Simultaneous detection of multiple mutation forms in liver cancer without capturing; 2. Suitable for efficient capture of ultra-small target regions; 3. The library may support 10-20 tests; 4. Ligate the DNA barcode to the starting ctDNA molecule during the library construction process, and cooperate with the biological information analysis process to achieve high specific detection of low-frequency mutations in ctDNA; 5. The library is usable for PCR hot spots detection and sequencing by a capture method at the same time, the added DNA barcode may effectively filter out false positive mutations and achieve high-specificity sequencing based on duplex. The disclosure helps with early screening, disease tracking, efficacy evaluation, prognosis prediction and the like of liver cancer.

Method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer

The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.

Method for rapidly constructing amplicon library through one-step process

Growing community of inventors

Beijing, China

Hai Yan