University of Nebraska

Hai Xuan

David S Hage

Chunling Wa

Abby Jackson

The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided.

Restricted access media and methods for making restricted access media

A method and kit for immobilization of a glycoprotein. The method may include activating an affinity support. The affinity support may be activated by reacting the affinity support with a compound that is reactive with one or more functional groups included within the glycoprotein. The method may also include oxidizing the glycoprotein in which oxidation conditions are selected to yield an oxidized glycoprotein that is biologically active and contains a sufficient number of reactive aldehyde groups for coupling to a support. For example, the oxidized glycoprotein may include five reactive aldehyde groups. In addition, the method may include reacting the oxidized-glycoprotein with the activated affinity support to immobilize the glycoprotein.

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Rolla, MO, United States of America

Hai Xuan