Sd Biosensor, Inc.

Other

Bioneer Corporation

Hae Joon Park

Sun Young Lee

Jae Young Kim

Dong Hun Kim

Hyo Lim Park

Young Shik Cho

Hyo Guen Lee

Kwan Hun Lim

In Ae Kim

Han Oh Park

Hyun Bae Kim

Jun Mo Gil

Seong Youl Kim

Yoo Deok Won

Mi Ae Lim

Hyo Jin Seong

The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene&#x2014;specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.

Isothermal based-dual functional oligonucleotide including reporter dye, and quencher for isothermal nucleic acid amplification and measurement methods using

There is provided a piston of a cartridge for extracting nucleic acids comprising: a cylindrical upper body having a hollow; a lower body having two ports; a control rod module combined to the other end of the upper body to seal the other end and move up and down along the hollow; and a rotation control module that is combined to the shaft of the lower body to transmit a driving force to the lower body.

Piston of nucleic acid extracting cartridge

There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

Method for extracting nucleic acid using cartridge

The present invention discloses a flow path structure of a cartridge for nucleic acid extraction comprising: a plurality of elongated grooves formed on the surface of the cartridge body; and a shaft hole formed in the center of the body, wherein the plurality of grooves have a starting point formed around the shaft hole and an ending point extending from the starting point toward the outer region of the body.

Channel structure of nucleic acid extracting cartridge

There is provided a cartridge for nucleic acid extraction comprising: a first body having a plurality of chambers in which ports are formed at the bottom; a second body coupled to a lower region of the first body; and a piston disposed rotatably in the centers of the first body and the second body and having a port formed at the bottom thereof; and characterized in that the cartridge comprises a plurality of flow paths formed on the upper region of the second body, one end overlapping the port of the piston and the other end overlapping the port of the first body.

Cartridge for extracting nucleic acid

The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

Primers for PCR amplification comprising a basic parts within the primer sequences

Growing community of inventors

Seongnam-si, South Korea

Hae Joon Park