Korea Research Institute of Bioscience and Biotechnology

Bioleaders Corporation

Ha Ryoung Poo

Moon-Hee Sung

Chul-Joong Kim

Seung Pyo Hong

Hyun-mi Pyo

Sue-nie Park

Jong-Soo Lee

Seung Goo Lee

Chang-Min Jung

Moon Hee Sung

Seong-Pyo Hong

Jong Soo Lee

Eun Ja Yoon

Chang Min Jung

Yoon Ho Choi

Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-&#x3c7;-glutamic acid synthetase complex (pgsBCA) of asp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

Vector for anti-HPV vaccine and transformed microorganism by the vector

Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-&#x3c7;-glutamic acid synthetase complex (pgsBCA) of asp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus, Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

An antibiotic-independent vector for stable vector maintenance and high protein expression and a method for gene expression using this vector are disclosed. The stable maintenance of the vector is due to expression of a glutamic acid racemase to complement a D-glutamic acid auxotroph. No antibiotics, such as ampicillin, are necessary for the stable maintenance of this vector.

Antibiotics-independent vector for constant high-expression and method for gene expression using the same

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Daejeon, South Korea

Ha Ryoung Poo