Bioleaders Corporation

Ha Na Moon

Young Chul Park

Se Eun Byeon

Dae Eun Ki

Hyun Jun Kang

Kyung Soo Hahm

Gyeong Jun Nam

Provided is a vector capable of co-expressing two different target proteins on the microbial surface using two promoters derived from, and a method of expressing target proteins on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism, and allows different foreign proteins to be stably expressed on the surface of the microorganism. Furthermore, provided is a surface expression vector containing the gene pgsA encoding a poly-gamma-glutamate synthetase complex, and a method of expressing a target protein on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism, and allows the foreign proteins to be stably expressed on the surface of the microorganism.

Surface expression vector using two kinds of promoters derived fromfor concurrently expressing two target proteins and method for expressing proteins on microbial surface by using same

Provided is a galactose mutarotase gene promoter derived fromand the use thereof, and more particularly, to a-derived galactose mutarotase gene promoter having the nucleotide sequence of SEQ ID NO: 1, an expression vector containing the promoter, and a microorganism transformed with the expression vector. A microorganism transformed with an expression vector containing the promoter may effectively express a target protein on the cell surface, and thus is useful as a vaccine vehicle or the like. Moreover, provided is a surface expression vector having pgsA, which is a gene encoding poly-gamma-glutamate synthetase, and a method of expressing a target protein on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism and allows a foreign protein is to be stably expressed on the surface of the microorganism.

Surface expression vector for constitutive high-expression using promoter of galactose mutarotase gene derived from, and use thereof

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Yongin-si, South Korea

Ha Na Moon