The Brigham and Women's Hospital, Inc.

Children's Medical Center Corporation

H William Harris

Edward M Brown

Steven C Hebert

Mark L Zeidel

Polycation-sensing receptors present in aquatic species and methods of regulating polycation-sensing receptor-mediated functions in aquatic species are described.

Polycation-sensing receptor in aquatic species and methods of use thereof

Polycation-sensing receptor in aquatic species and methods of use

A method for determining the diuretic potency of candidate drugs as inhibitors of ADH-elicited water channels is disclosed. In a preferred embodiment, such diuretic potency of candidate drugs is determined by suspending vesicles containing ADH water channels in an aqueous suspending medium, incorporating the candidate into the suspension, rapidly changing the pH of the suspending medium and thereafter detecting the proton permeability of the water channels. The proton permeability in the presence of the candidate drug can then be compared to the proton permeability of water channels under similar conditions without the presence of the candidate drug to determine the diuretic potency of the candidate drug to inhibit ADH-elicited water channels.

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Dover, MA, United States of America

H William Harris