University of Virginia Patent Foundation

Zygem Corporation Limited

Lockheed Martin Corporation

Leidos Innovations Technology, Inc.

Microlab Diagnostics, Inc.

H Randall Bell

James P Landers

Daniel J Marchiarullo

John W Pettit

Orion N Scott

Joan Marie Bienvenue

Brian Root

Daniel C Leslie

Jessica V Norris

Jessica Norris

Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.

DNA analyzer

Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.

Optical approach for microfluidic DNA electrophoresis detection

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Arlington, VA, United States of America

H Randall Bell