Mycogen Corporation

Dow Agrosciences LLC

Hycogen Corporation

H Ernest Schnepf

Kenneth Edwin Narva

George E Schwab

Brian A Stockhoff

Mark E Knuth

Jewel M Payne

Guy A Cardineau

Michael R Pollard

Tracy Ellis Michaels

Judy Muller-Cohn

Stacey Finstad Lee

Lisa M Stamp

Luis Foncerrada

Jerald S Feitelson

John B Randall

Charles Joseph Dullum

James Schmeits

David Loewer

Gregory A Bradfisch

M Keith Kennedy

Leo Kim

George Morrill

Stacey Finstad-Lee

Joanna Dojillo

Carol Wicker

Michele Walz

August J Sick

Mark Thompson

Steven Lee Evans

Heidi J Uick

Jenny M Fu

Henry Meier

Mikki Walz

Blake Sturgis

Rod Aaron Herman

Paula Diehl

Paula Burmeister

Frank H Gaertner

Raymond J Cannon

David O Brower

Lisa M Morera

This invention provides modified, insecticidal Cry34 proteins with enhanced properties as compared to wild-type Cry34 proteins. The modifications to these proteins were based in part on an analysis of the three-dimensional (3D) structure of this protein and other proteins in the Cry34 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry34 proteins.

Plants and polynucleotides encoding truncated Cry34 proteins

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This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the &#x2dc;45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

Modified Cry35 proteins

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Pesticidal proteins

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal protein have molecular weights of approximately 40&#x2013;50 kDa and of approximately 10&#x2013;15 kDa.

The subject invention concerns the discovery ofisolates with advantageous activity against weevils. In preferred embodiments of the invention,isolates, or toxins therefrom, are used to control alfalfa weevils, boll weevils, and/or rice water weevils. The toxins can be administered to the pests through a variety of methods including the transformation of bacteria or plants to produce the weevil-active toxins.

The subject invention provides KB59A4-6 pesticidal proteins and preferred methods of using these proteins to control lepidoteran pests. This invention providesisolate KB59A4-6.

Pesticidal proteins and methods of using these proteins

Disclosed and claimed are novel toxins and genes obtainable fromisolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.

Pesticidal toxins and genes from Bacillus laterosporus strains

The subject invention concerns new classes of insecticidal proteins obtainable from, and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.

Pesticidal toxins

The subject invention concerns materials and methods useful in the control of non-mammalian pests and, particularly, plant pests. In a specific embodiment, the subject invention provides new toxins useful for the control of lepidopterans. The subject invention further provides nucleotide sequences which encode the toxins of the subject invention. The nucleotide sequences of the subject invention can be used to transform hosts, such as plants, to express the pesticidal toxins of the subject invention. The subject invention further concerns novel nucleotide primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxin-encoding genes.

Toxins active against pests

Growing community of inventors

San Diego, CA, United States of America

H Ernest Schnepf