Dna Genotek Inc.

H Chaim Birnboim

Adele Jackson

Roy Sunstrum

Rafal Michal Iwasiow

Rod Muir

Paul Lem

Michael Beach

Romeo Graham

Mike G Sirois

Derek Kirkland

Ian Curry

Olle Maarten De Bruin

Rama Nadia Panford-Walsh

Ellen Joan Maclean

Jonathan David Liberty

Evgueni Vladimirovitch Doukhanine

Carlos Alberto Merino Hernandez

Joanne Chartier

Bitapi Ray

Maria Mercedes Acero

Maurice Lavimodiere

Jacques Oscar Everard Niles

Cassandra Kelly-Cirino

Ellen Joan MacLean

Jonathan D Liberty

Ellen Maclean

Jacques Oscar Niles

The present application is to provide a composition and method for stabilizing and maintaining the viability of hardy microorganisms from sample collection to downstream analysis. In particular, there is a method for preserving viable hardy bacteria, such as, or, in a biological sample, comprising contacting the biological sample with a stabilization composition, wherein the stabilization composition comprises a chelating agent, a denaturing, a salt and has a pH between about 6 and about 11.

Composition and method for stabilizing and maintaining the viability of hardy microorganisms

The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.

Compositions and methods for obtaining nucleic acids from sputum

The present application provides a composition comprising periodates or persulfates for extracting nucleic acid from a microorganism, and a corresponding method of using the composition for nucleic acid extraction. The composition and method are particularly useful in extracting nucleic acid from microorganisms that are generally resistant to standard nucleic extraction techniques, such as, one or more species of thegenus, one or more species of thecomplex, MDR strains of, one or more species of, one or more species of, and other microorganisms with hardy cell walls.

Method and system for microbial lysis using periodates

The present invention provides a container system for releasably storing a substance. The container system includes a vial having a sample storage chamber and a piercing member for piercing a membrane in the lid, which membrane seals a substance within a reservoir in the lid until the membrane is pierced by the piercing member. The container system optionally includes a funnel. There is also provided a method and kit for use of such a container system.

Container system for releasably storing a substance

Sample collection device

A sample collection tool comprises a collection end configured for collecting a sample. Optionally, the sample is collected from within a body cavity of an animal without causing significant discomfort or injury to the animal. A first handle portion has a first end connected to the collection end and a second end. A second handle portion is connected to the second end of the first handle portion by a breakable connection. The second end of the first handle portion has at least one winglet. The at least one winglet is compliant in a preferential direction. The breakable connection is ruptured at least in part when the at least one winglet is forced in the preferential direction.

Sample collection tool

A closure for a container has a closure body having a first cylindrical portion and a second cylindrical portion opposite the first portion. The closure also has a connector disposed in at least one of the first and second portions. The connector is adapted to connect one of a sample collection device and an applicator to the closure. A containing apparatus including the closure, a container, and optionally one of a sample collection device and an applicator is also disclosed. A method of using the containing apparatus is also disclosed.

Closure, containing apparatus, and method of using same

The present invention provides an aqueous composition and method for extracting nucleic acid from a sample of bodily fluid, such as saliva, such that the nucleic acid within said sample remains stable for at least fourteen days at room temperature. The composition permits direct use of the extracted and stored DNA in an amplification reaction without further processing.

Compositions and method for storage of nucleic acid from bodily fluids

Saliva collection tube

Growing community of inventors

Ottawa, Canada

H Chaim Birnboim