Korea Advanced Institute of Science and Technology

Gyun Min Lee

Gou Young Koh

Chan Soo Kim

Sun Chang Kim

Ho Min Kim

Young Jun Koh

Keehoon Jung

Jung-Eun Lee

Sun Ok Hwang

Joo Young Chung

No Soo Kim

The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and placenta growth factor (PIGF) polypeptide comprising a nucleotide sequence encoding a VEGFR1 component.

Glycosylated VEGF decoy receptor fusion protein

The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and TNF polypeptide comprising: (a) a nucleotide sequence encoding a TNFR2 component and VEGFR1 component operatively linked to (b) a nucleotide sequence encoding a multimerizing component, wherein the TNFR2 component consists essentially of a nucleotide sequence encoding the amino acid sequences of cystein rich domain 1, cystein rich domain 2, cystein rich domain 3, and cystein rich domain 4 of the extracellular domain of TNFR2, and wherein the VEGFR1 component consists essentially of a nucleotide sequence encoding the amino acid sequences of Ig-like domain 2 of the extracellular domain of VEGFR1.

Fusion protein capable of binding VEGF-A and TNF-alpha

The present invention relates to a method for mass-production of target protein, particularly to a method for mass-production of trombopoietin by regulating the expression of chaperone protein.

Method for mass-production of target protein by regulating the expression of chaperone protein

The present invention relates to an antisense nucleotide of caspase-3, an expression vector encoding antisense RNA of caspase-3, and an inhibition method of cellular apoptosis in recombinant Chinese hamster ovary (CHO) cells using the same. More precisely, the present invention relates to an antisense nucleotide composed of a base sequence represented by Sequence ID No. 1, which suppresses the expression of caspase-3, an expression vector containing the above antisense nucleotide and expressing antisense RNA of caspase-3, and an inhibition method of apoptosis of recombinant cells by suppressing the expression of caspase-3 through introducing the above expression vector into the recombinant cells and then expressing antisense RNA of caspase-3. According to the present invention, apoptosis can be inhibited by suppressing the activation of caspase-3, which is related to the apoptosis, and the integrity of target protein produced in the recombinant cells can be enhanced.

Inhibition of apoptosis by the expression of antisense RNA of caspase-3

Growing community of inventors

Daejeon, South Korea

Gyun Min Lee