Wisconsin Alumni Research Foundation

Scarab Genomics, LLC

Campbell, John W.

Guy Plunkett

Frederick R Blattner

Gyorgy Posfai

John Walter Campbell

Christopher D Herring

Jeremy D Glasner

David A Frisch

Valerie Burland

Nicole T Perna

Rod Welch

Trevor Twose

György Posfai

Trevor Martin Twose

Reduced genome strains ofMGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.

[object Object]

The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Prophage element-free bacteria

Reduced genome strains ofMG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.

A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.

Insertion sequence-free bacteria

The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Bacteria with reduced genome

The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.

Methods and compositions for producing recombinant proteins using a gene for TRNA

The various embodiments of the present invention relate generally to plasmid DNA preparations and to methods for producing and using such preparations.

Plasmid DNA preparations and methods for producing same

The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specificstrains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.

Competent bacteria

The entire genome of pathogenicstrain 0157:H7 has been sequenced. All of the genomic DNA sequences present in 0157 and absent in the previously sequenced laboratory strain K12 are presented here.

Sequences of E. coli O157

The entire genome of pathogenic strain O157:H7 has been sequenced. All of the genomic DNA sequences present in O157 and absent in the previously sequenced laboratory strain K12 are presented here.

Growing community of inventors

Madison, WI, United States of America

Guy Plunkett