Innogenetics N.v.

Fujirebio Europe N.v.

Guy Mersch

Rudi Rossau

Geert Jannes

Ilse De Canck

Kurt Troch

The present invention relates to the typing of HLA alleles. The sequence of exon 2 and exon 3 of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51new and of exon 2 of the alleles HLA-DRB1*0820, HLA-DRB1*04new and HLA-DRB4*01new are disclosed. The present invention relates to methods for typing of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51new. The present invention further provides primers and probes to be used in the methods for typing. A diagnostic kit comprising these primers and probes is also part of the present invention.

Method for typing HLA alleles

The present invention relates to the typing of HLA alleles. The sequence of exon 2 and exon 3 of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51new and of exon 2 of the alleles HLA-DRB1*0820, HLA-DRB1*04new and HLA-DRB4*01new are disclosed. The present invention also relates to methods of typing of such alleles. According to a particular embodiment, typing of alleles is achieved by: i) amplifying a relevant fragment of the alleles with at least one suitable set of primers; ii) hybridizing the amplification product of step i) to at least one probe that specifically hybridizes to a target region comprising one or more polymorphic nucleotides in said relevant fragment; and iii) determining from the result of step ii) the absence or presence of the alleles in the sample. The present invention further provides primers and probes to be used in such methods of typing alleles. Diagnostic kits comprising such primers and probes are also included within the invention.

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Ghent, Belgium

Guy Mersch