Agracetus

Monsanto Technology LLC

Gurdip S Brar

Oliver Nelson

Gail A Roberts

The present invention relates to improved methods for producing transgenic sunflower plants. Specifically, methods are disclosed for transforming sunflower tissues and regenerating fertile transgenic plants therefrom. The methods are particularly amenable to sunflower tissues, such as cotyledons, which have proven refractory to previous methods of transformation and regeneration.

Transformation and regeneration of sunflower cotyledons

A method is disclosed for efficiently using genic male-sterile maize in hybrid seed production which allows economical maintenance of male-sterile stocks. This method enhances a natural linkage between a selected male-sterile gene and a marker gene conditioning an easily observable trait by deletion and screening to create stocks in which the linkage is effectively complete.

Genic male-sterile maize using a linked marker gene

A method is disclosed for creating and utilizing genic male-sterile maize for hybrid maize production. The method makes use of the Spm transposable element system and involves creation of a mutated male fertile allele, mutated by the presence of the Spm suppression receptor therein, expression of which is suppressed in the presence of the Spm suppressor. Methods are disclosed for transferring this system into any inbred line of interest for use in hybrid seed production.

Growing community of inventors

Middleton, WI, United States of America

Gurdip S Brar