Jiangsu Academy of Agricultural Sciences

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Snu Rdb Foundation

Guoqing Shao

Zhixin Feng

Qiyan Xiong

Yanfei Yu

Beibei Liu

Lei Zhang

Li Wang

Rong Chen

Jongwoo Lim

Yanna Wei

Yuan Gan

Jia Wang

Yuzi Wu

Yun Bai

Daesub Song

Maojun Liu

Long Zhao

Fei Hao

Minjoo Yeom

Lulu Xu

Wenliang Li

Yongjie Liu

Xing Xie

A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 10TCID/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.

Viral vector delivery system for both respiratory and digestive tracts of pigs and application thereof

A composition and a kit for detectingare provided. The composition for detectingis an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5' terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of

Composition and kit for detecting mycoplasma

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Nanjing, China

Guoqing Shao