Shaanxi University of Science and Technology

Guoli Gong

Jian Zha

Zhiqiang Liu

Runcong Sun

A method for extracting protein without lysing cells includes: step 1: combining a penetrating peptide CPP gene having a sequence as set forth in SEQ ID NO: 1 and a bacterial lyase T4L gene having a sequence as set forth in SEQ ID NO: 2 through a flexible connecting peptide GGGGS gene having a sequence as set forth in SEQ ID NO: 3 to form a fusion enzyme CPP-T4L gene having a sequence as set forth in SEQ ID NO: 4; step 2: inserting the fusion enzyme CPP-T4L gene, and obtaining a recombinant host strain; step 3: cloning a target protein expression gene, and then constructing a recombinant expression strain; step 4: first inducing the expression of the target protein expression gene; starting the expression of the fusion enzyme CPP-T4L gene, and releasing a target protein; step 5: collecting cell lysis supernatant to recover the target protein.

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Xi'an, China

Guoli Gong