Tsinghua University

Capitalbio Corporation

Capitalbio Technology Corporation

Guohao Zhang

Jing Cheng

Wanli Xing

Lei Wang

Can Wang

Guoliang Huang

Jian Gao

Yao Zhang

Yang Wang

Jiang Zhu

Xinying Zhou

Juan Xin

Mingxian Lin

Hongju Guo

Zhengde Feng

Su Guo

In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.

Integrated microfluidic device for target amplification and microarray detection

A multi-index detection microfluidic chip is provided. A bottom plate () and a cover plate () that matches the bottom plate () and seals it are provided in the microfluidic chip. The center of the microfluidic chip has a through-hole (). The bottom plate () has one or more wave-shaped main channel(s) (), one end of each of the main channel () connected to a sample injection hole () on the bottom plate (), and the other end connected to an exhaust hole () on the bottom plate (). The valley on the main channel () is distal to the through-hole (), and the peak is proximal to the through-hole (). Each valley of the main channel () is connected to a reaction chamber () by a linking channel (). The linking channel () comprises one or more buffering chambers (). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction.

Multi-index detection microfluidic chip and methods of use

Provided is a microfluidic chip, which comprises a substrate and a cover sheet, wherein a microreactor array is arranged on the substrate and comprises at least one main channel () and at least two micro cells () connected to the main channel (). The microfluidic chip also comprises at least one local temperature control device, which is used for heating the main channel () or cooling the micro cells (). The use of the microfluidic chip ensures uniformity and independency of the micro cells (). Also provided is an application of the microfluidic chip in biological detection or medical inspection.

Microfluidic chip and application thereof

Growing community of inventors

Beijing, China

Guohao Zhang