Hoechst Aktiengesellschaft

Gunther J Riess

Reinhard Kirsch

Laszlo Vertesy

Irvin Winkler

Manfred Rosner

Klaus-Peter Koller

Jorg-Peter Kleim

Joachim Wink

Jozsef Aszodi

Wilhelm Stahl

Dieter Isert

Martin Knauf

Dominique Le Beller

Eugen Uhlmann

Karl Geisen

Werner Aretz

Klaus Sauber

Christoph Meichsner

Holger Wallmeier

Uta-Maria Billhardt-Troughton

Bernd Rosenstock

Substituted quinoline derivatives with antiviral action

This invention relates to the antibiotic feglymycin, as well as processes for its preparation and use. Feglymycin is represented by the structural formula (SEQ. ID. NO. 1) ##STR1##

Methods of treatment with novel antibiotic feglymycin

This invention relates to the antibiotic feglymycin, as well as processes for its preparation and use. Feglymycin is represented by the structural formula (SEQ ID NO:1): ##STR1##

Antibiotic, feglymycin, processes for its preparation and its use

The gene encoding glutarylacylase (GA) contained in the plasmid pCM 145 (DSM 6409) permits the expression of GA in E. coli in high yields.

Process for the preparation of glutarylacylase in large quantities

Compounds of the formula I, ##STR1## and also their tautomeric forms of the formula Ia, ##STR2## in which the substituents R.sup.1 to R.sup.6 and X have said meanings, exhibit antiviral activity.

Substituted quinoline derivatives, a process for their preparation, and

Disclosed are quinoxalinone compounds of the formula I or Ia ##STR1## and physiologically tolerated salts and prodrugs thereof, in which n=zero, one or two;

Quinoxalines, a process for their preparation and their use

New insulin derivatives, a process for their preparation and use, and a pharmaceutical formulation containing them are disclosed. The derivatives contain the basic amino acid arginine at the amino-terminal position of the insulin A-chain, and various amino acid substitutions at the carboxyl terminus of the insulin B-chain. The compounds are suitable for the treatment of diabetes mellitus, have a delayed profile of action and are very well tolerated.

Insulin derivatives, a process for the preparation thereof, the use

The tendamistat gene can be used for the construction of fused genes with which fusion proteins are expressed and excreted in Streptomycetes host cells. The tendamistat portion can be modified, in particular it can be C-terminal shortened. When a gene for a shortened proinsulin in which the insulin B chain is linked to the A chain only via Lys or Lys-Lys is coupled to the tendamistat gene, this gene construction is introduced into an expression vector, and the latter is used to transform a Streptomycetes host cell, there is expression and secretion of the corresponding fusion protein. The fusion protein can easily be cleaved to give insulin precursors because of correctly established disulfide bonds. Genetic structures which code for the signal sequence and about the first ten amino acids of tendamistat as well as a desired protein are expressed in streptomyces host cells with a high yield, and the fusion proteins are secreted into the medium.

Processes for the preparation of foreign proteins in streptomycetes

A tandem arrangement of two promoters which are each active in Streptomyces is provided. The tandem arrangement provides a considerable increase in protein expression.

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Frankfurt am Main, Germany

Gunther J Riess