Boehringer Mannheim Gmbh

Gunter Frey

Dieter Berger

Manfred Kuhr

Wolfgang Werner

Gerd Zimmermann

Franz Braun

Wolfgang-Reinhold Knappe

Werner Guthlein

Walter Rittersdorf

Joachim Siedel

Hans-Rudiger Murawski

Doris Horn

Elke Biegel

Test carrier for determining an analyte in whole blood

The present invention is concerned with the use of certain 3-aminopyrazolo-heterocyclic compounds for the colorimetric determination of hydrogen peroxide, hydrogen peroxide-forming systems, peroxidase, peroxidate-active substances and electron-rich aromatic compounds. The present invention is also concerned with corresponding processes of determination and with agents appropriate therefor. Furthermore, the present invention is concerned with new 3-aminopyrazolo-heterocyclic compounds.

3-aminopyrazolo heterocyclic derivatives, and use for colorimetric

The present invention is concerned with aniline derivatives of the general formula: ##STR1## wherein R.sub.1 is hydrogen or a lower alkyl or aryl, R.sub.2 is hydrogen or a lower alkyl which can be substituted by hydroxyl, amino carboxyl, lower alkoxycarbonyl, lower alkanoylamide lower alkyl or aryl or by a group of the structure ##STR2## is which R.sub.5 and R.sub.6 have the meanings given hereinafter, R.sub.3 is a hydrogen or halogen atom or a carboxyl group, R.sub.4 and R'.sub.4, which can be the same or different, are hydrogen or halogen, carboxyl or lower alkoxy or lower alkyl radicals which are preferably in the m-position, R.sub.5 is hydrogen or a lower alkyl and R.sub.6 is hydroxyl or a lower alkoxy, lower alkyl or aryl radical; and of the salts thereof with acids and bases useful as a color former in the Trinder reaction for the detection of hydrogen peroxide or hydrogen peroxide-forming systems or peroxidase or peroxidate-acting substances or together with an oxidation agent for the detection of aromatic amines or of systems forming such amines.

Aniline derivatives useful as color formers

The present invention is concerned with the use of aniline derivatives of the general formula: ##STR1## wherein R.sub.1 is a hydrogen atom or a lower alkyl or aryl radical, R.sub.2 is a hydrogen atom or a lower alkyl radical which can be substituted by hydroxyl, amino, carboxyl, lower alkoxycarbonyl, lower alkanoylamido, aryl lower alkyl or aryl or by a group of the structure: ##STR2## in which R.sub.5 and R.sub.6 have the meanings given hereinafter, R.sub.3 is a hydrogen or halogen atom or a carboxyl group, R.sub.4 and R'.sub.4, which can be the same or different, are hydrogen or halogen atoms, carboxyl groups or lower alkoxy radicals or lower alkyl radicals which are preferably in the m-position and R.sub.1 and R.sub.4 or R.sub.2 and R.sub.4 or R.sub.4 and R'.sub.4, when the two substituents are ortho to one another, can together form a saturated or unsaturated hydrocarbon chain containing 2 to 6 carbon atoms which can be substituted by hydroxyl or oxo groups, R.sub.5 is a hydrogen atom or a lower alkyl radical and R.sub.6 is a hydroxyl group or a lower alkoxy, lower alkyl or aryl radical; and of the salts thereof with acids and bases

Agent and process for the detection of redox systems

A test support for support-bound tests for the determination of components of fluids, especially of human and animal body fluids. The test support is composed of a plurality of layers, and the joining of the layers is accomplished at least partially by sewing.

Test carrier

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom and the method of preparation and use.

Agent for the determination of esterolytic and/or proteolytic enzymes

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom; and the method of preparation and use.

This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.

Amino acid and peptide esters of leuko-indoaniline compounds and

Azo dyestuff for use as a chromogen in detecting leukocytes

Diagnostic agent for the detection of leukocytes in body fluids, comprising an absorbent carrier which is impregnated with a chromogen and a buffer substance, wherein the chromogen is a sulfonphthalein ester of the formula ##STR1## wherein R.sub.1 is a carboxylic acid residue optionally substituted by halogen or lower alkoxy; or is an amino acid or peptide residue having a nitrogen protective group;

Diagnostic agent for the detection of leukocytes and chromogens useful

This invention relates to diagnositc agents and methods for the detection of leukocytes in body fluids. In additional aspect, the invention provides a process for the production of such diagnostic agents.

Diagnostic agents for the detection of leukocytes in body fluids

Novel diagnostic composition for the detection of leukocytes in body fluids, comprising an absorbent carrier impregnated with a buffer substance and a chromgen, wherein the chromogen is an azo dyestuff ester of the formula

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Ellerstadt, Germany

Gunter Frey