Gyros Ab

Gyros Patent Ab

Gunnar Kylberg

Per X Andersson

Owe Salven

Gunnar Thorsen

Mats Inganas

Mats Inganäs

Gunnar Thorsén

Gunnar Thors N

A microscale method for the characterization of one or more reaction variables that influence the formation or dissociation of an affinity complex comprising a ligand and a binder, which have mutual affinity for each other. The method is characterized in comprising the steps of: (i) providing a microfluidic device comprising a microchannel structures that are under a common flow control, each microchannel structure comprising a reaction microactivity; (ii) performing essentially in parallel an experiment in each of two or more of the plurality of microchannel structures, the experiment in these two or more microchannel structures comprising either a) formation of an immobilized form of the complex and retaining under flow conditions said form within the reaction microactivity, or b) dissociating, preferably under flow condition, an immobilized form of the complex which has been included in the microfluidic device provided in step (i), at least one reaction variable varies or is uncharacterized for said two or more microchannel structures while the remaining reaction variables are kept essentially constant; (iii) measuring the presentation of the complex in said reaction microactivity in said two or more microchannel structures; and (iv) characterizing said one or more reaction variables based on the values for presentation obtained in step (iii).

Characterization of reaction variables

Method for determining the amount of an analyte with a disc-shaped microfluidic device

A method of controlled heating of a micro channel reactor structure () comprises providing a structure (b, b, B, B) defining a desired temperature profile. A preferred embodiment of a heating element structure comprises a pattern of areas of a material capable of providing heat when energized, disposed over said micro channel reactor structure.

Device and method for the controlled heating in micro channel systems

A microfluidic device which comprises two or more microchannel structures (set), each of which comprises a structural unit which comprises (i) one or more inlet microconduits, and (ii) an outlet microconduit downstream said one or more inlet microconduits, and (iii) a flow path for a liquid passing through either of said inlet microconduits and said outlet microconduit. The device is characterized in that each outlet microconduit in said two or more microchannel structures is a restriction microconduit. There may also be a microcavity between the inlet microconduit(s) and the restriction microconduit in each microchannel structure. Typically common flow control is used for driving a liquid flow within the device. The innovative design is useful for creating flow with low inter-channel variation with respect to the microchannel structures of the device.

Functional unit enabling controlled flow in a microfluidic device

An apparatus for performing temperature cycling, comprising a micro channel reactor structure (), and having a heating structure (bbBB) defining a desired temperature profile. A preferred embodiment of a heating element structure comprises a pattern of areas of a material capable of providing heat when energized, disposed over said micro channel reactor structure.

Growing community of inventors

Bromma, Sweden

Gunnar Kylberg