Bristol-myers Squibb Compnay

Guna Romancik

John J Usher

Michael Politino

Sean M Tonzi

David A Lowe

William V Burnett

The present invention relates to a novel D-hydantoinase from that enantio-selectively hydrolyzes D-hydantoins to their corresponding D-N-carbamoyl-amino acids; nucleic acids that encode for the enzyme; expression vectors including the nucleic acids; and host cells capable of expressing the enzyme.

D-hydantoinase from Ochrobactrum anthropi

Disclosed is a process for the synthesis of .beta.-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said process.

Synthesis of .beta.-lactam antibacterials using soluble side chain

Precursors of .beta.-lactam antibacterials having soluble side chain

The present invention is concerned with a chemical acetylation process for the preparation of 3'-acetylated cephalosporin from desacetyl-cephalosporin. The novel process is carried out with an acetyl donor and an activator or catalyst.

Chemical acetylation of desacetyl-cephalosporins

The instant invention provides nucleic acid molecules encoding cephalosporin esterase from Rhodosporidium toruloides. In addition, the invention provides isolated cephalosporin esterase, expression vectors, host cells, and methods of producing cephalosporin esterase.

Cephalosporin esterase gene from Rhodosporidium toruloides

O-acylated cephalosporins are produced by reacting 3-hydroxymethylcephalosporins with an acyl donor containing at least three carbon atoms and an enzyme which is a Rodosporidium toruloides esterase, a wheat germ lipase, an Aspergillus niger lipase, an orange peel acetylesterase or a Bacillus subtilis esterase. A preferred enzyme is the esterase from Rodosporidium toruloides ATCC 10657. The enzyme can be used while in whole cells or in soluble or inmobilized form.

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Jamesville, NY, United States of America

Guna Romancik