Green Cross Holdings Corporation

Gun Sul Lee

Ki-Yong Kim

Dong-Hwan Park

Yong Gyu Kang

Sung Min Choi

Kang Yun Seo

Ki-Hwan Son

Dong-Hwarn Park

Jun Sic Kim

Ki Hwan Son

Yong Kang

A method for preparing a highly concentrated fibrinogen solution includes adding amino acid or amino acid derivatives, and/or salts to a lowly concentrated fibrinogen solution, followed by a ultra-filtration concentration. Factor XIII can be added either before or after the ultra-filtration to give a fibrin sealant componentcontaining the highly concentrated fibrinogen solution. The fibrin sealant componentcould be preserved for a long time at room temperature and be used without a reconstitution. Fibrin sealant componentis a solution containing thrombin and calcium. A fibrin sealant product may be provided in a vial type in which the fibrin sealant componentsandare each filled in separate vials or in a re-filled syringe type wherein the fibrin sealant componentsandare each filled in separate syringes connected with each other to be instantly used.

Method for preparing highly concentrated fibrinogen solution and method for preparing fibrin sealant by using thereof

The present invention relates to a method for purifying an immunoglobulin, and more particularly, to a method for purifying an immunoglobulin, which comprises: dissolving immunoglobulin-containing plasma protein fraction I+II+III or fraction II+III; adding caprylate to the solution to cause precipitation; performing dialysis and concentration after removal of the precipitate; performing anion exchange resin and ceramic cation exchange resin purification processes to effectively remove a solvent and detergent added to inactivate viruses; and performing elution while maintaining salt concentration at a constant level to maintain the immunoglobulin polymer content at a low level. According to the method for preparing the intravenous immunoglobulin according to the present invention, a precipitation step of preparing fraction II from fraction I+II+III or fraction II+III as a starting material can be omitted, and problems, including a complicated process and a low yield, which occur in the conventional preparation process employing the polyethylene glycol treatment process, can be solved by use of first sodium caprylate precipitation, anion exchange chromatography and cation exchange chromatography. In addition, when the immunoglobulin purification method according to the present invention is used, the efficiency with which impurities and thrombotic substances are removed can be increased and the immunoglobulin polymer content can be maintained, and thus a stable immunoglobulin with increased quality can be produced.

Method for purifying immunoglobulin

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Suwon-si, South Korea

Gun Sul Lee