Chinese Academy of Sciences

Institute of Genetics and Developmental Biology, Cas

Guifu Liu

Jiayang Li

Jing Wang

Qian Qian

Yonghong Wang

Bing Wang

Mingjiang Chen

Yanhui Jing

Yongqing Jiao

Xiangbing Meng

Hong Yu

Dawei Xue

Huihui Liu

Guojun Dong

The disclosure discloses a method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution, and a use of the multisubunit SCF E3 ligase. The method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution disclosed by the disclosure includes: subjecting an engineered SKP1-Cullin1-RBX1 fusion protein (referred to as eSCR) to a reaction with an F-box protein in a reaction system to obtain the multisubunit SCF E3 ligase, where the eSCR fusion protein has an amino acid sequence from position 10 to position 1062 of SEQ ID NO: 2. Experimental results show that different multisubunit SCF E3 ligases are successfully prepared with the eSCR fusion protein through in vitro reconstitution in the disclosure; the reconstituted multisubunit E3 ligase has biological activity.

Method for preparing multisubunit SCF E3 ligase with fusion protein through in vitro reconstitution, and use of multisubunit SCF E3 ligase

The present invention discloses a protein IPA1 related to plant architecture, its coding genes and uses, wherein the protein is 1) or 2) as follows: 1) the protein consisting of the amino acid sequence showed by sequence 1 in the sequence list; 2) the protein derived from the protein of 1) by substitution and/or deletion and/or addition of one or several amino acid residues in the amino acid sequence defined in 1) and relating to plant architecture. IPA1 gene can be used for molecular marker-assisted breeding, cultivating new rice varieties and improving rice yield.

Protein IPA1 related to plant architecture, its coding genes and uses

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Beijing, China

Guifu Liu