Chinese Academy of Sciences

Guibin Jiang

Qian Jack Liu

Guangbo Qu

Jianbo Shi

Yanna Liu

Qianchi Ma

Xiu Huang

A metabolic enzyme-induced micro-nano plastic particle biodegradation method and a product analysis method therefor are disclosed. The biodegradation method includes the following steps: step (1), cutting an appropriate quantity of plastic products into small pieces; step (2), placing the plastic products in a ball mill tank and milling the plastic products in vacuum in a ball mill; step (3), weighing and dispersing the milled plastic sample with water to prepare a dispersion with a concentration; step (4), mixing the micro-nano plastic dispersion with a metabolic enzyme solution, and placing the mixed solution in a vortex oscillator for mixing well; and step (5), incubating the mixed solution in a waterproof incubator. The present invention provides for the first time a method for biodegradation and metabolism of micro-nano plastics under mild conditions and under the induction of a metabolic enzyme, namely, glutathione S-transferase.

Metabolic enzyme-induced micro-nano plastic particle biodegradation method and product analysis method therefor

Machines and methods are for performing fully-automated biological evaluation and chemical analysis. A pretreatment module is used in treatments of enriching, concentrating and purifying a sample to be analyzed. A component separation module is used for carrying out separation of multiple compounds in mother liquor to be analyzed. A monitoring and identifying module is for monitoring and collecting chromatographic signals of the separated liquid effluent in real time, as well as quantitative detection of suspicious compounds. A component collection module is used in operations of collecting, transferring and dissolving, redissolving, and pipetting the separated liquid effluent. A biological evaluation module is for cell culture and detection of cytotoxic effect and toxic targets. A data processing and automated control module is for acquisition, arrangement and analysis of the integrated data. The machines and methods provide an efficient, stable and normalized standard operation condition for the screening of toxicity of compounds.

Integrated machines and methods for performing fully-automated biological evaluation and chemical analysis

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Beijing, China

Guibin Jiang