Wacker Chemie Ag

Guenter Wich

Tobias Dassler

Susanne Leonhartsberger

Walfred Leinfelder

Simone Mitterweger

The invention relates to a method for producing L-cysteine and its derivatives L-cystine and thiazolidine by fermenting a cysteine-producing microorganism strain in a fermentation medium. The invention is characterized in that L-methionine, L-isoleucine, or L-threonine in respective concentrations ranging from 0.1 to 10 g/L are added to the fermentation medium in the main culture.

Method for the fermentative production of L-cysteine and derivatives of said amino acid

The present invention relates to a process for producing a correctly folded and assembled full-length antibody using anstrain, which comprises fermenting anstrain which leaks periplasmic proteins into the medium, comprising a gene coding for the heavy chain of an antibody functionally linked to a signal sequence coding for a signal peptide, and a second gene coding for the light chain of an antibody, functionally linked to a signal sequence coding for a signal peptide, in a culture medium, where thestrain secretes a full-length antibody into the culture medium, and the full-length antibody is removed from the culture medium.

Process for the fermentative production of antibodies

A process for producing a heterologous protein is provided. The process comprises culturing anstrain in a fermentation medium, thestrain harboring a gene which codes for the heterologous protein and is functionally linked to a signal sequence coding for a signal peptide, and releasing the heterologous protein into the fermentation medium, and the heterologous protein being removed from the fermentation medium, wherein thestrain has an attenuated (p)ppGpp-synthetase II activity (PSII activity).

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A microorganism strain comprises a gene coding for a recombinant protein and a mutated gene coding for a host protein that is not a protease. The recombinant protein is secreted during a fermentation and the mutated gene coding for the host protein has been mutated so as to cause reduced expression of the host protein compared to the wild-type gene on which the mutated gene is based.

Growing community of inventors

Munich, Germany

Guenter Wich