Albert-Ludwigs-Universitaet Freiburg

Biocopy Gmbh

Hahn-Schickard-Gesellschaft Fuer Angewandte Forsch

Robert Boschgmbh

Guenter Roth

Jochen Hoffmann

Roland Zengerle

Martina Daub

Nils Paust

Juergen Steigert

Felix Von Stetten

Beate Strehlitz

Bernd Faltin

Christine Reinemann

Arne Kloke

Marion Finkbeiner

Johannes Woehrle

Simon Wadle

Stefan Daniel Kraemer

The invention relates to a method for microarray transformation, wherein, by using a cavity chip with transformation matrix, a template array can be copied onto a planar support, and the information or spatial arrangement is changed in the process, so that a transformed second array forms. The invention further relates to a device for carrying out such a method.

Microarray transformer

The invention relates to a mediator probe comprising a probe region and a mediator region. Furthermore, the invention relates to a system comprising a mediator probe and a detection molecule, use of that system and a method for detection of at least one target molecule.

Bifunctional oligonucleotide probe for universal real time multianalyte detection

The invention relates to a method for performing a biochemical or chemical reaction for an isolated, spatially separated amplification of sample fragments during a simultaneous immobilization and spatial arrangement of the sample fragments and reaction products, the amplification products, on one or more suitable solid phases for subsequent derivatizations.

Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations

The invention relates to a method for identifying aptamers, having the following steps &#x2014;bringing a mixture of oligonucleotides into contact with an aptamer target structure and binding at least some of the oligonucleotides to the target structure, &#x2014;separating the oligonucleotides which have been bound to the aptamer target structure from the aptamer target structure and from oligonucleotides that are not bound to the aptamer target structure, &#x2014;amplifying individual oligonucleotides which were bound to the aptamer target structure in a physically separate manner and producing a plurality of physically separate amplicons, each amplicon predominantly containing one type of oligonucleotides, &#x2014;specifying a specific marker for a plurality of the physically separate amplicons such that each of the marked amplicons can be uniquely identified using the specified marker of the amplicon, &#x2014;sequencing oligonucleotides in a plurality of marked amplicons and assigning the marker that is specific for the amplicon to the sequence of the type of oligonucleotides in the amplicon for each amplicon examined by means of the sequencing process &#x2014;analyzing the binding properties of the types of oligonucleotides to the aptamer target structure and assigning the analyzed binding properties to the specific markers of the amplicons and to the sequences of the types of oligonucleotides.

Method for identifying aptamers

The invention relates to a method for detecting at least one target molecule and/or product molecule, wherein reaction components are provided in defined regions of a base surface and/or covering device. At least two defined regions with different reaction components are provided, and an amplification reaction is carried out. The invention also relates to a device for carrying out said method.

Multiplexed digital PCR

A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.

Device and method for producing a replicate or derivative from an array of molecules, and applications thereof

The invention relates to a method for producing a microarray, wherein the production of this array is detected in real time from the accumulation of the product molecules being produced. The invention further relates to a microarray produced by this method, and to a device for the real-time detection of molecular accumulations on an array surface during the production of microarrays.

Device and method for real-time detection of molecular accumulations and/or monitoring the production process of a molecular microarray

A device for insertion into a rotor of a centrifuge has at least two bodies stacked one above the other in a stacking direction inside a housing insertable into a holder of the rotor of the centrifuge. The two bodies are movably disposed in relation to each other inside the housing in order to fluidically couple, in a first phase, responding to a rotation of the rotor, a first cavity of the first body to a first cavity of the second body and to fluidically couple in a second phase a second cavity of the first body to the first cavity of the second body.

Growing community of inventors

Freiburg, Germany

Guenter Roth