Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Helmholtz Zentrum Munchen Deutsches Forschungszentrum Fuer Gesundheit Und Umwelt

Guenter R Fuhr

Heiko Zimmermann

Rolf Hagedorn

Charli Kruse

Julia Neubauer

Thomas Schnelle

Bernd Wagner

Andreas Voigt

Stefan Fiedler

Frank Stracke

Jan Hornung

Henning Glasser

Torsten Mueller

Robert Lemor

Philipp Ciba

Dirk Leuthold

Marianne Belke

Uwe Schoen

Klaus-Peter Hoffmann

Axel Beier

Daniel Doerr

Bernd Kranz

Tomm Schmidt

The invention relates to a low temperature indicator mixture for indicating/monitoring a temperature transition within a temperature range of between −20° C. and −160° C., which comprises at least one dye, e.g. oil red, methyl rot, brilliant green, rhodamine B, and at least one alcohol selected from the group that comprises octane-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butane-2-ol, pentane-1,5-diol, pentane-1-ol, cyclopentanol, benzyl alcohol, wherein the melt temperature of the mixture is within the aforementioned temperature range. Further aspects of the invention relate to devices and methods for indicating/monitoring a temperature transition within the aforementioned temperature range, in which devices and methods the aforementioned low temperature indicator mixture is used. In a specific embodiment, a device of this kind comprises at least the following components in at least one closed space: a) a partial region, which is at least partially filled with an indicator mixture as defined above; b) a second partial region, which is at least partially filled with an absorbent material, said material being optionally separated from the indicator mixture by a separating element; and c) optionally means to penetrate the separating element to bring the indicator mixture in a liquid state into contact with the absorbent material. In a method according to the invention for temperature monitoring using a device of this kind, a check is carried out to determine whether at least part of the indicator mixture was absorbed by the absorbent material, which indicates an at least temporary increase in the temperature of the device to at least the melt temperature of the indicator mixture.

Low temperature indicator mixture, device and method for monitoring a temperature transition at low temperatures

The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container (in particular a cryotube) having a holding space for holding a biological sample and comprising at least one chamber, the interior of which is not fluidically connected to the holding space and is filled only partially with an indicator substance, the melting temperature of which lies in a range of &#x2212;20&#xb0; C. to &#x2212;140&#xb0; C. In particular, the chamber can be formed by a container that is detachably or pivotably fastened to the sample container. Alternatively, the chamber is formed by a double-walled slide-on part or the holding space of the sample container is double-walled, wherein an intermediate space between the inner wall and the outer wall is partially filled with the indicator substance.

Method and device for the temperature monitoring of a cryopreserved biological sample

The invention relates to a method for monitoring the temperature of a cryopreserved biological sample. The invention also relates to a device for monitoring the temperature of a cryopreserved biological sample. The device () for monitoring the temperature of a cryopreserved biological sample comprises a sample container () having a receiving space () for receiving a biological sample (). The device also comprises at least one chamber () having an interior that is not fluidically connected to the receiving space () and is only partially filled with an indicator substance () with a melting temperature in a region of &#x2212;20&#xb0; C. to &#x2212;140&#xb0; C. The chamber () has a barrier () that causes the indicator substance () to move into a second sub-region () of the chamber () when the indicator substance () in a first sub-region () of the chamber is in the fluid aggregate state.

Device and method for monitoring the temperature of a cryopreserved biological sample

The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container having an accommodating space (2) for accommodating the sample and comprising an indicating apparatus, which can be arranged on the outside of the sample container, for monitoring at least one temperature limit value. The indicating apparatus has at least one cavity, which is only partially filled with an indicating substance, the melting temperature of which lies in a range from &#x2212;20 &#xb0; C. to &#x2212;140 &#xb0; C. The indicating apparatus can be designed, in particular, as a cylindrical body, which can be fastened to a cryotube as a bottom part, or alternatively as a double-walled hollow cylinder, which can be slid onto an outer lateral surface of the cryotube. The indicating apparatus can also be fastened to a lateral outer wall of the sample container, e.g. as a hollow body that can be inserted into a sleeve or insertion pocket.

The invention relates to a device for monitoring the temperature of a cryogenically preserved biological sample. The device () comprises a sample container () having a receptacle space () for accommodating a biological sample (). The device further comprises at least one chamber (), the interior () of which is not fluidically connected to the receptacle space, and which is at least partially filled with an indicator substance () having a boiling point or sublimation temperature in a range from &#x2212;10&#xb0; C. to &#x2212;140&#xb0; C. The chamber () further has at least one opening via which the indicator substance () can escape from the interior () of the chamber () upon exceeding its boiling point or sublimation point. The invention further relates to a method for monitoring the temperature of a cryogenically preserved biological sample.

Device and method for monitoring the temperature of a cryogenically preserved biological sample

The invention relates to a temperature-control element () for a multiwell plate (), which comprises a plurality of cavities () arranged in rows and columns for freezing and/or thawing biological samples. The temperature-control element () comprises a base body () which is made of a thermally conductive material and is flown through by a temperature-control fluid; and a plurality of protruding temperature-control fingers () arranged in rows and columns on an upper side of the base body (), which are connected in a thermally conductive manner to the base body (), wherein a grid spacing of the temperature control fingers () corresponds to a grid spacing of the cavities () of the multiwell plate (). The invention further relates to a device and method for freezing biological samples, in particular for cryopreservation, and/or thawing biological samples, in particular a cryopreserved sample.

Temperature-control element for a multiwell plate and method and device for freezing and/or thawing biological samples

A culture vessel (), which is designed for culturing biological cells () in hanging drops (), comprises a vessel wall (), which has a cover portion () and a floor portion (), wherein the cover portion () is designed to provide a culturing surface (), and the floor portion () is designed to receive a liquid (), the vessel wall () encloses an interior () of the culture vessel on all sides, the culturing surface () has holding elements (), which are designed to position the drops (), wherein the culturing surface () can receive the drops () hanging freely in the interior (), and the vessel wall () is movable such that the holding elements () can be wetted with the liquid () from the floor portion (). A method for culturing biological cells () in hanging drops () in the culture vessel () is also described.

Culture vessel and method for culturing biological cells in hanging drops

The invention relates to a method for monitoring a cryopreserved biological sample (), comprising the steps of providing the biological sample () in a cryopreserved state, measuring at least one Raman spectroscopic sample characteristic by means of a Raman spectroscopic measuring apparatus (), comparing the at least one sample characteristic with a reference characteristic by means of an evaluating apparatus (), and providing a state characteristic that depends on the result of the comparison and that is characteristic of a storage state of the biological sample (). The invention further relates to a monitoring device for cryopreserved samples, in particular for performing said method.

Method and device for monitoring a cryopreserved biological sample

A substrate unit for cryopreservation of a biological sample includes: a substrate platform having on a front side thereof a cultivation surface for receiving the biological sample; a first chamber including the cultivation surface of the substrate platform, wherein the first chamber is configured for receiving a cultivation liquid; a second chamber configured for receiving a temperature control medium; and a chamber bracket for receiving the substrate unit in a cryopreservation device in a pivotable manner, wherein the first and second chambers are connected to each other in an adjacent manner, and the substrate platform forms a separating wall between the first chamber and the second chamber, and wherein the back side of the substrate platform faces the second chamber. The invention further relates to a cryopreservation device, wherein the substrate unit is arranged in a rotatable manner, and a method for the cryopreservation of a biological sample.

Substrate unit, preservation device and method for the cryopreservation of a biological sample

The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct () for delivering a coolant () to a cooling chamber (), a heater () that has an adjustable first heating performance (P) for heating the coolant () delivered to the cooling chamber (), a first temperature sensor (-) for measuring the temperature (T-T) in the cooling chamber (), a second temperature sensor () for measuring the temperature (Ti) of the coolant () delivered to the cooling chamber (I), and a regulator () for regulating the temperature. The regulator () is embodied as a multiple regulator which detects several temperatures (T-T) as control variables and/or adjusts several heating performances (P, P) as manipulated variables. The invention further relates to a corresponding operating method.

Cooling apparatus used for cryonic preservation, and corresponding operating method

A protective suit (), especially for an operator () in a cooling chamber that is cooled using liquid nitrogen or vapor of the liquid nitrogen comprises a body suit () which has a thermally insulating, gas-tight cover material () and is designed to accommodate the operator (), and a heating device () which is connected to the body suit () and is designed to heat the interior of the protective suit (). A glove () which is made of a thermally insulating glove material () and includes a glove heater () is also described.

Protective suit for use in a cooling chamber

A cooling system (), especially for cryopreserving biological samples (), comprises a cooling chamber () delimited by a bottom area (), side walls (), and a top area (), and a cooling device () for cooling the cooling chamber () using liquid nitrogen (). At least one of the side walls () includes at least one predetermined wall element () which is a portion of at least one of the side walls and can be moved relative to the associated side wall () in such a way that a wall opening can be formed in said side wall (). Methods for operating the cooling system () in case of an emergency are also described.

Cooling system, especially for cryopreserving biological samples, comprising devices for use in case of an emergency

The invention relates to a deep-sea device for recovering at least one deep-sea object, in particular at least one organism and/or cell material. The deep-sea device comprises a capturing system () for accommodating at least one deep-sea object, and a drive unit () for driving the deep-sea device. The deep-sea device is designed and equipped in particular to be advanced at least in sections substantially in the buoyancy direction (Ar) toward the water surface. The deep-sea device is designed in particular like a torpedo.

Deep-sea device for recovering at least one deep-sea object

Disclosed is a deposition apparatus () which is designed to deposit frozen biological material () in a target substrate () and includes a charging device () and a driving device (). The charging device () is designed to supply the biological material () to the driving device () while the driving device () is designed to apply a driving force to the biological material (). In order to apply the driving force, the driving device () is formed such that the biological material () can penetrate into the target substrate () under the effect of the driving force. Also disclosed are an injector cartridge which is designed to provide frozen biological material in a deposition apparatus as well as a method for depositing biological material () in a target substrate ().

Apparatus and method for the deposition of biological material in a target substrate

The invention relates to a cryogenic storage device and to a method for operating a cryogenic storage device, particularly for cryogenic storage of biological samples, comprising a sample carrying device that is set up to accommodate samples, a cryogenic container that is set up to accommodate the sample carrying device, a container cooling device with which the cryogenic container can be cooled, an intermediate storage container that is set up for intermediate storage of the sample carrying device, a transport device with which the sample carrying device can be moved between the cryogenic container and the intermediate storage container and a sensor device with which at least one operating parameter of the cryogenic storage device can be registered. The transport device is set up to be activated in dependence on an output signal from the sensor device upon registering a predetermined operating condition of the cryogenic storage device, so that the sample carrying device is moved from the cryogenic container to the intermediate storage container. Additionally or alternatively, an intermediate storage cooling device that is set up for cooling the intermediate storage container can be activated in dependence on an output signal of the sensor device.

Growing community of inventors

Berlin, Germany

Guenter R Fuhr