Carl Zeiss Jena Gmbh

Guenter Berthel

Hans-Juergen Dobschal

Dirk Muehlhoff

Thomas Hartmann

Stefan Schmidt

Dieter Graefe

Gerhard Doering

Wolfgang Grau

Gunther Elender

Guenther Rudolph

A method and an arrangement for detecting the position of the plane XY of an object, which plane XY is to be scanned, and for its positioning in the focal plane X&prime;Y&prime; of a laser scanner, preferably for a laser scanning microscope. According to the disclosure, it is provided in a method of the type described above that, after a rough orientation of the object carried out by placing on an object holder, a laser beam is directed successively in time to at least three different points P , P . . . P located in the scan plane XY of the object and, in doing so, each of the reflections proceeding from the points P , P . . . P is imaged on a position-sensitive detector, an actual position value is determined at the detector for each reflection and is compared with a stored reference position value, adjustment commands for changing the inclination of the object holder are obtained from the deviations of the actual position values from the reference position values, and the inclination of the object holder is changed on the basis of these adjustment commands until points P , P . . . P are located in the focal plane X&prime;Y&prime; of the laser scanner.

Method and array for detecting the position of a plane scanned with a laser scanner

An arrangement for the detection of biomolecular reactions and interactions using the RS screening method and comprises a specimen plate or microtiter plate (PP or MTP), a white light source with subsequently arranged illumination beam path, a splitter plate on which the PP or the MTP is set for generating a detection beam path, and optical means for imaging the measurement beam path and reference beam path on a spatially resolving detector array of a CCD camera which is connected with an evaluating unit or with a computer for determining measurement values. The arrangement particularly provides optical imaging elements for imaging the measurement beam path and reference beam path at different positions on the detector array of the CCD camera simultaneously and without overlap, a monochromator which comprises a plurality of interference filters and which is arranged subsequent to the light source in the illumination beam path; a component assembly which collimates the illumination beam path, is formed of optical elements and is arranged between the monochromator and the splitter plate; and specimen plate or microtiter plate, and an optical imaging element for complete spatal separation of the measurement beam path and reference beam path in, or in the vicinity of, an intermediate inage plane or aperture diaphragm plane.

Arrangement for detecting biomolecular reactions and interactions

Growing community of inventors

Jena, Germany

Guenter Berthel