Lonza Cologne Gmbh

Amaxa Ag

Lonza Cologne Ag

Gudula Riemen

Gregor Siebenkotten

Juliana Helfrich

Elke Lorbach

Heike Wessendorf

Helmut Brosterhus

Corinna Thiel

Meike Weigel

Kirsten Rothmann-Cosic

Michael Nix

Herbert Mueller-Hartmann

Ludger Altrogge

Herbert Müller-Hartmann

Rainer Christine

Katharina Hein

Bodo Ortmann

Tatjana Males

Marion Gremse

Dietmar Lenz

Hanns-Martin Schmidt

Tamara Grabek

Andrea Mueller-Hartmann

Günter Kraus

Kirsten Rothmann

Helmut Brosterbus

Tamara Turbanski

Andreas Klaes

Sabine Poppenborg

Andrea Klaes

A method for introducing biologically active molecules into animal or human cells using an electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution including HEPES and at least 10 mmol&#xd7;1magnesium ions (Mg), the buffer solution having a buffer capacity of at least 20 mmol&#xd7;1&#xd7;pHat a change in the pH from pH 7 to pH 8 and at a temperature of 25&#xb0; C., and an ionic strength of at least 200 mmol&#xd7;1. An electric voltage is applied to the suspension.

Buffer solution for electroporation and a method comprising the use of the same

The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.

Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current

A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce the biologically active molecules into the cells using electric current. The buffer solution includes at least one of sodium succinate, mannitol and sodium lactobionate. The buffer solution has a buffer capacity of at least 20 mmol*l*pHat a change in the from pH 7 to pH 8 and at a temperature of 25&#xb0; C., and an ionic strength of at least 200 mmol * l.

A method for introducing biologically active molecules into animal or human cells using electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution which has a buffer capacity of at least 20 mmol&#xd7;l&#xd7;pHand an ionic strength of at least 200 mmol&#xd7;lat a change in the pH from pH 7 to pH 8 and at a temperature of 25&#xb0; C. to form a suspension. The method further includes applying an electric voltage to the suspension so as to introduce the biologically active molecules into animal or human cells.

The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.

Modular transfection systems

Disclosed is a device for processing biological material which at least comprises a chamber at least closable to the outside and having an inner space for receiving the biological material. The chamber comprises at least one electrode placed in contact with the inner space for generating an electric field. Also disclosed is a method for processing biological material. The biological material is introduced into the inner space above and the electrode can generate an electric field after said biological material is introduced by applying voltage to said electrode and a further electrode in contact with the inner space. The chamber comprises at least one inlet line having at least one opening arranged close to the electrode. The biological material is almost completely rinsed out of the inner space after the electric field is generated, via a solution guided via an inlet line of the chamber along at least one electrode.

Device and method for processing biological material

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol&#xd7;pHand an ionic strength of at least 200 mmol&#xd7;1during a change to the pH value from pH 7 to pH 8 and at a temperature of 25&#xb0; C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.

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Langenfeld, Germany

Gudula Riemen