Boehringer Mannheim Gmbh

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Innogenetics N.v.

Gudrun Schmitz-Agheguian

Waltraud Ankenbauer

Frank Laue

Christine Ebenbichler

Vitaly Svetlichny

Elizaveta Bonch-Osmolovskaya

Bernhard Angerer

Christoph Kessler

Michael Jarsch

Klaus Kaluza

Rudi Rossau

Gregor Sagner

Thomas Emrich

Bruno Frey

Geert Jannes

Astrid Reiser

Gerd Haberhausen

Ursula Markau

Hans J Hoeltke

Martin Moczko

Daniel De Vos

A purified thermostable enzyme is derived form the thermophilic archaebacterium. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95&#xb0; C. in the presence of stabilizing agents and possesses 3'-5&#x2032; proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

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A purified thermostable enzyme is derived from the thermophilic archaebacteriumThe enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95&#xb0; C. in the presence of stabilizing agents and possesses 3'-5&#x2032; proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3&prime;-5&prime; exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.

Thermostable DNA polymerase from carboxydothermus hydrogenoformans

Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:

Type II restriction endonucleae MCRI

The type II restriction endonuclease RleAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Rhizobium. It can be used to recognize and cleave the double-stranded DNA sequence CCCACA(N).sub.12/9 and its complementary sequence.

Type II restriction endonuclease RleAI

The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.

Type II restriction endonuclease SgrAI

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Bernried, Germany

Gudrun Schmitz-Agheguian