Boehringer Mannheim Gmbh

Gudrun Schmitz

Christoph Kessler

Michael Jarsch

Ralf Mattes

Rudolf Seibl

Bryan J Bolton

Hans-Joachim Holtke

Hans Robert Scholer

Waltraud Ankenbauer

Frank Laue

Klaus Kaluza

For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.

Process for the detection of nucleic acids

The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.

Process for the reduction of star activities

Type II restriction endonuclease MamI

The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.

Class II restriction endonuclease KspI and a process for obtaining it

The present invention provides a restriction endonuclease, characterized by the recognition sequence:

Class II restriction endonuclease Ksp632I, a process for obtaining it

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Bernried, Germany

Gudrun Schmitz