University of Utah Research Foundation

Biofire Defense, LLC

Idaho Technology, Inc.

Biofire Diagnostics, LLC

Gudrun Reed

Carl Thomas Wittwer

Luming Zhou

Virginie E Dujols

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Growing community of inventors

Salt Lake City, UT, United States of America

Gudrun Reed